Any feedback?
Literature summary for 4.1.1.32 extracted from
- Structures of rat cytosolic PEPCK: insight into the mechanism of phosphorylation and decarboxylation of oxaloacetic acid (2007), Biochemistry, 46, 10078-10088.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Rattus norvegicus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapour diffusion method, at 25 ?C in 0.1 M HEPES (pH 7.4) and 12-30% PEG 3350, creating PEPCK-Mn2+, PEPCK-Mn2+-oxaloacetate, PEPCK-Mn2+-oxaloacetate-Mn2+GDP, and PEPCKMn2+-Mn2+GTP crystals | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Rattus norvegicus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P07379 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione-Uniflow resin column chromatography and P6DG column chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + oxaloacetate | - |
Rattus norvegicus | GDP + phosphoenolpyruvate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PEPCK | - |
Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
