Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL-21 | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mechanism of inactivation of PEPCK by Cys-273 modification due to inhibition of a dynamic motion that may occur upon nucleotide binding | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic isoform | Rattus norvegicus | 5829 | - |
mitochondrion | mitochondrial isoform | Gallus gallus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | binds to enzyme more weakly than Mn2+ | Rattus norvegicus | |
Mn2+ | binds to the active site, activates | Gallus gallus | |
Mn2+ | binds to the active site, activates | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
1 * 67000 | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + oxaloacetate | Gallus gallus | primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis | GDP + phosphoenolpyruvate + CO2 | - |
? | |
GTP + oxaloacetate | Rattus norvegicus | primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis | GDP + phosphoenolpyruvate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
recombinant enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + oxaloacetate | specifically requires GTP or ITP, structural data of GTP binding | Gallus gallus | GDP + phosphoenolpyruvate + CO2 | - |
r | |
GTP + oxaloacetate | specifically requires GTP or ITP, structural data of GTP binding | Rattus norvegicus | GDP + phosphoenolpyruvate + CO2 | - |
r | |
GTP + oxaloacetate | primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis | Gallus gallus | GDP + phosphoenolpyruvate + CO2 | - |
? | |
GTP + oxaloacetate | primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis | Rattus norvegicus | GDP + phosphoenolpyruvate + CO2 | - |
? | |
ITP + oxaloacetate | specifically requires GTP or ITP | Gallus gallus | IDP + phosphoenolpyruvate + CO2 | - |
r | |
ITP + oxaloacetate | specifically requires GTP or ITP | Rattus norvegicus | IDP + phosphoenolpyruvate + CO2 | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Rattus norvegicus |
monomer | 1 * 67000 | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
GTP/ITP: oxaloacetate carboxylase (transphosphorylating) | - |
Gallus gallus |
GTP/ITP: oxaloacetate carboxylase (transphosphorylating) | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gallus gallus |
25 | - |
assay at | Rattus norvegicus |