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Literature summary for 4.1.1.32 extracted from

  • Vial, M.V.; Oelckers, K.B.; Rojas, M.C.; Simpfendrfer, R.W.
    Purification, partial kinetic characterization and reactive sulfhydryl groups of the phosphoenolpyruvate carboxykinase from Perumytilus purpuratus adductor muscle (1995), Comp. Biochem. Physiol. B, 112, 451-460.
No PubMed abstract available
Search for more literature for 4.1.1.32

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Perumytilus purpuratus
ITP
-
 Perumytilus purpuratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 IDP pH 7, in presence of 2.3 mM Mn2+ Perumytilus purpuratus
0.55
-
 phosphoenolpyruvate pH 7, in presence of 2.3 mM Mn2+ Perumytilus purpuratus
2.4
-
 CO2 pH 7, in presence of 2.3 mM Mn2+ Perumytilus purpuratus
2.4
-
 CO2 CO2 in form of HCO3- Perumytilus purpuratus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent metal ion required, decreasing order of effectiveness: Co2+, Mn2+, Zn2+, Mg2+ Perumytilus purpuratus
Mg2+ divalent metal ion required, decreasing order of effectiveness: Co2+, Mn2+, Zn2+, Mg2+ Perumytilus purpuratus
Mn2+ divalent metal ion required, decreasing order of effectiveness: Co2+, Mn2+, Zn2+, Mg2+ Perumytilus purpuratus
Zn2+ divalent metal ion required, decreasing order of effectiveness: Co2+, Mn2+, Zn2+, Mg2+ Perumytilus purpuratus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 1 * 74000, SDS-PAGE Perumytilus purpuratus
85000
-
 gel filtration Perumytilus purpuratus

Organism

Organism UniProt Comment Textmining
Perumytilus purpuratus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Perumytilus purpuratus

Source Tissue

Source Tissue Comment Organism Textmining
adductor
-
 Perumytilus purpuratus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13
-
-
 Perumytilus purpuratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + phosphoenolpyruvate + CO2
-
 Perumytilus purpuratus GTP + oxaloacetate
-
 r
GTP + oxaloacetate
-
 Perumytilus purpuratus GDP + phosphoenolpyruvate + CO2
-
 ?
IDP + phosphoenolpyruvate + CO2
-
 Perumytilus purpuratus ITP + oxaloacetate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 74000, SDS-PAGE Perumytilus purpuratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
-
 Perumytilus purpuratus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6: about 50% of maximal activity, pH 8: about 25% of maximal activity Perumytilus purpuratus