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Literature summary for 4.1.1.31 extracted from

  • Muramatsu, M.; Suzuki, R.; Yamazaki, T.; Miyao, M.
    Comparison of plant-type phosphoenolpyruvate carboxylases from rice identification of two plant-specific regulatory regions of the allosteric enzyme (2015), Plant Cell Physiol., 56, 468-480 .
    View publication on PubMed
Search for more literature for 4.1.1.31

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-bisphosphate
-
 Oryza sativa Japonica Group
D-glucose 1-phosphate
-
 Oryza sativa Japonica Group

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure Oryza sativa Japonica Group

Protein Variants

Protein Variants Comment Organism
additional information replacement of the N-terminal 25 residues of Ppc2a with the 27 N-terminal residues of chloroplastic isoform Ppc4 reduces Vmax value to 75% and markedly affects the sensitivities to aspartate and glutamate. When K119 at the N-terminus of the N-terminal loop is replaced with the glutamate-alanine pair of isoform Ppc4, K119EA mutant, S0.5 for phosphoenolpyruvate of the resulting Ppc2a mutant increases 4fold relative to that of the wild type and reaches the level observed for isoform Ppc4 Oryza sativa Japonica Group
S12D mutation of phosphorylatable serine Oryza sativa Japonica Group

Inhibitors

Inhibitors Comment Organism Structure
D-fructose 1,6-bisphosphate
-
 Oryza sativa Japonica Group
L-aspartate very low sensitivities to allosteric inhibitors aspartate and glutamate; very low sensitivities to allosteric inhibitors aspartate and glutamate Oryza sativa Japonica Group
L-glutamate very low sensitivities to allosteric inhibitors aspartate and glutamate; very low sensitivities to allosteric inhibitors aspartate and glutamate Oryza sativa Japonica Group
L-malate
-
 Oryza sativa Japonica Group

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 phosphoenolpyruvate wild-type, S0.5 value, Hill coefficient 1.63, pH 8.0, 30°C Oryza sativa Japonica Group
0.18
-
 phosphoenolpyruvate N-terminal replacement mutant, S0.5 value, Hill coefficient 1.77, pH 7.3, 30°C Oryza sativa Japonica Group
0.19
-
 phosphoenolpyruvate wild-type, S0.5 value, Hill coefficient 1.52, pH 7.3, 30°C Oryza sativa Japonica Group
0.21
-
 HCO3- wild-type, S0.5 value, pH 7.3, 30°C Oryza sativa Japonica Group
0.33
-
 HCO3- wild-type, S0.5 value, pH 7.3, 30°C Oryza sativa Japonica Group
0.56
-
 phosphoenolpyruvate wild-type, S0.5 value, Hill coeffcient 2.39, pH 8.0, 30°C Oryza sativa Japonica Group
0.64
-
 phosphoenolpyruvate wild-type, presence of glucose 6-phosphate, S0.5 value, Hill coeffcient 1.84, pH 7.3, 30°C Oryza sativa Japonica Group
0.74
-
 phosphoenolpyruvate wild-type, S0.5 value, Hill coeffcient 2.06, pH 7.3, 30°C Oryza sativa Japonica Group
0.76
-
 phosphoenolpyruvate K119EA mutant, presence of glucose 6-phosphate, S0.5 value, Hill coefficient 2.00, pH 7.3, 30°C Oryza sativa Japonica Group
0.79
-
 phosphoenolpyruvate mutant S12D, presence of glucose 6-phosphate, S0.5 value, Hill coeffcient 1.64, pH 7.3, 30°C Oryza sativa Japonica Group
0.83
-
 phosphoenolpyruvate mutant S12D, S0.5 value, Hill coeffcient 1.10, pH 7.3, 30°C Oryza sativa Japonica Group

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Oryza sativa Japonica Group 9507
-
cytosol
-
 Oryza sativa Japonica Group 5829
-

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group
-
-
-
Oryza sativa Japonica Group Q8H4V1
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf produced at high levels in leaf mesophyll cells Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + HCO3-
-
 Oryza sativa Japonica Group phosphate + oxaloacetate
-
 ?

Synonyms

Synonyms Comment Organism
OJ1484_G09.129-1
-
 Oryza sativa Japonica Group
PPC2a
-
 Oryza sativa Japonica Group
PPC4
-
 Oryza sativa Japonica Group

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.03
-
 pH 7.3, 30°C Oryza sativa Japonica Group L-malate
0.07
-
 pH 7.3, 30°C Oryza sativa Japonica Group L-aspartate
0.29
-
 pH 8.0, 30°C Oryza sativa Japonica Group L-malate
0.35
-
 pH 7.3, 30°C Oryza sativa Japonica Group L-malate
0.39
-
 pH 7.3, 30°C Oryza sativa Japonica Group L-glutamate
7.78
-
 pH 7.3, 30°C Oryza sativa Japonica Group L-glutamate
12.6
-
 pH 8.0, 30°C Oryza sativa Japonica Group L-malate
13.2
-
 pH 7.3, 30°C Oryza sativa Japonica Group L-aspartate