Application | Comment | Organism |
---|---|---|
synthesis | multienzyme system composed of recombinant phosphoenolpyruvate, in combination with mesophilic/thermophilic bacterial carbonic anhydrases, for converting CO2 into oxaloacetate. The catalytic procedure is in two steps: the conversion of CO2 into bicarbonate by carbonic anhydrase, followed by the carboxylation of phosphoenolpyruvate with bicarbonate, catalyzed by PEPC, with formation of oxaloacetate. When coupled with the extremely thermostable carbonic anhydrase from Sulphurhydrogenibium azorense, the production of oxaloacetate is achieved even at temperatures up to 60°C | Thermosynechococcus vestitus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
phosphoenolpyruvate | pH 8.3, 23°C | Thermosynechococcus vestitus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosynechococcus vestitus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + HCO3- | - |
Thermosynechococcus vestitus | phosphate + oxaloacetate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
15 min, no loss of activity | Thermosynechococcus vestitus |
50 | - |
15 min, 84% residual activity | Thermosynechococcus vestitus |
60 | - |
15 min, 71% residual activity | Thermosynechococcus vestitus |