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Literature summary for 4.1.1.31 extracted from
- Improvement of the phosphoenolpyruvate carboxylase activity of Phaeodactylum tricornutum PEPCase 1 through protein engineering (2014), Enzyme Microb. Technol., 60, 64-71.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells | Phaeodactylum tricornutum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.169 | - |
HCO3- | at pH 8.0 and 25°C | Phaeodactylum tricornutum |  |
| 0.79 | - |
phosphoenolpyruvate | at pH 8.0 and 25°C | Phaeodactylum tricornutum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Phaeodactylum tricornutum | 5829 | - |
| mitochondrion | - |
Phaeodactylum tricornutum | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 117000 | - |
x * 117000, recombinant enzyme, SDS-PAGE | Phaeodactylum tricornutum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + HCO3- | Phaeodactylum tricornutum | - |
phosphate + oxaloacetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaeodactylum tricornutum | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41.7 | - |
at pH 8.0 and 25°C | Phaeodactylum tricornutum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + HCO3- | - |
Phaeodactylum tricornutum | phosphate + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 117000, recombinant enzyme, SDS-PAGE | Phaeodactylum tricornutum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PEPCase1 | - |
Phaeodactylum tricornutum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | the enzyme remains stable | Phaeodactylum tricornutum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Phaeodactylum tricornutum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Phaeodactylum tricornutum | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 52.7 | - |
phosphoenolpyruvate | at pH 8.0 and 25°C | Phaeodactylum tricornutum | |
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