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Literature summary for 4.1.1.31 extracted from
- High activity and stability of codon-optimized phosphoenolpyruvate carboxylase from Photobacterium profundum SS9 at low temperatures and its application for in vitro production of oxaloacetate (2014), Bioprocess Biosyst. Eng., 37, 331-335.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli SGJS117 | Photobacterium profundum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 99000 | - |
x * 99000, SDS-PAGE | Photobacterium profundum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphate + oxaloacetate | Photobacterium profundum | - |
phosphoenolpyruvate + HCO3- | - |
? |  |
| phosphate + oxaloacetate | Photobacterium profundum SS9 | - |
phosphoenolpyruvate + HCO3- | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Photobacterium profundum | - |
- |
- |
| Photobacterium profundum SS9 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| agarose resin column chromatography | Photobacterium profundum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphate + oxaloacetate | - |
Photobacterium profundum | phosphoenolpyruvate + HCO3- | - |
? | |
| phosphate + oxaloacetate | - |
Photobacterium profundum SS9 | phosphoenolpyruvate + HCO3- | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 99000, SDS-PAGE | Photobacterium profundum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PEPC | - |
Photobacterium profundum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 40 | enzyme activity remains steady in the range of 5-30°C and rapidly declines in the range of 30-40°C | Photobacterium profundum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 10 | the enzyme shows high stability in the range of pH 8.5-10.0, while displaying no activity below pH 4.0 and at pH 11.5-13.0 | Photobacterium profundum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Photobacterium profundum | |
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Release 2023.1 (January 2023)
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