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Literature summary for 4.1.1.31 extracted from

  • OLeary, B.; Rao, S.K.; Kim, J.; Plaxton, W.C.
    Bacterial-type phosphoenolpyruvate carboxylase (PEPC) functions as a catalytic and regulatory subunit of the novel class-2 PEPC complex of vascular plants (2009), J. Biol. Chem., 284, 24797-24805.
    View publication on PubMed
Search for more literature for 4.1.1.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Ricinus communis

Inhibitors

Inhibitors Comment Organism Structure
D-fructose 6-phosphate 99% residual activity at 2 mM Ricinus communis
D-glucose 1-phosphate 95% residual activity at 2 mM Ricinus communis
D-glucose 6-phosphate 93% residual activity at 2 mM Ricinus communis
glycerol 3-phosphate 98% residual activity at 2 mM Ricinus communis
L-aspartate 98% residual activity at 2 mM Ricinus communis
L-malate 84% residual activity at 2 mM Ricinus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.84
-
 phosphoenolpyruvate at pH 7.3 Ricinus communis
0.87
-
 phosphoenolpyruvate at pH 8.0 Ricinus communis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely dependent on, PPC4 exhibits a 4fold higher specific activity with saturating (10mM) Mg2+ relative to Mn2+ Ricinus communis
Mn2+ may replace Mg2+ Ricinus communis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
 PPC4, gel filtration Ricinus communis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ricinus communis recombinant PPC4 forms class-2 PEPC when combined with class-1 PEPCs ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ricinus communis A6YM33 PPC4
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Superose-6 gel filtration, and Superdex 200 gel filtration Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Ricinus communis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
 clarified extract Ricinus communis
25.2
-
 after 152fold purification Ricinus communis

Storage Stability

Storage Stability Organism
-20°C, 50% (v/v) glycerol, the final PPC4 preparation proves difficult to store as it slowly loses activity and is completely inactivated when rapidly thawed after freezing in liquid N2 Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information recombinant PPC4 forms class-2 PEPC when combined with class-1 PEPCs Ricinus communis ?
-
 ?
phosphoenolpyruvate + CO2
-
 Ricinus communis phosphate + oxaloacetate
-
 ir

Synonyms

Synonyms Comment Organism
bacterial-type PEPC
-
 Ricinus communis
BTPC
-
 Ricinus communis
PEPC
-
 Ricinus communis
Phosphoenolpyruvate carboxylase
-
 Ricinus communis
PPC
-
 Ricinus communis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Ricinus communis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
11
-
 at pH 7.0 Ricinus communis L-malate
33
-
 at pH 7.0 Ricinus communis L-aspartate