Cloned (Comment) | Organism |
---|---|
cloned as a Histag-fusion protein in an Escherichia coli PEPC- (Ppc-) strain | Sorghum bicolor |
Protein Variants | Comment | Organism |
---|---|---|
delataC1 | deletion of the last c-terminal amino acid. kcat: 9.5/sec (phosphoenolpyruvate),12.6% of wild type catalytic activity | Sorghum bicolor |
delataC4 | deletion of the last 4 c-terminal amino acids. kcat: 0.174/sec (phosphoenolpyruvate), 0.14% wild type catalytic activity | Sorghum bicolor |
G961A | kcat: 17.4/sec (phosphoenolpyruvate), 24.3% of wild type catalytic activity | Sorghum bicolor |
G961V | kcat: 7.2/sec (phosphoenolpyruvate), 8.5% of wild type catalytic activity | Sorghum bicolor |
additional information | it is shown that even a modest, neutral alteration of the PEPC C-terminal hydrogen atom side chain is detrimental to enzyme function | Sorghum bicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
SDS-PAGE | Sorghum bicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | P15804 | sorghum C4 isoform | - |
Purification (Comment) | Organism |
---|---|
affinity purification | Sorghum bicolor |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Sorghum bicolor | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the four C-terminal mutant enzymes display varying degrees of PEPC activity in vitro ranging from 23% of wild-type with the modest G961A substitution to only 0.2% for the DELTAC4-truncated form | Sorghum bicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + CO2 | - |
Sorghum bicolor | phosphate + oxaloacetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PEPC | - |
Sorghum bicolor |
Phosphoenolpyruvate carboxylase | - |
Sorghum bicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sorghum bicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.174 | - |
phosphoenolpyruvate | DELTAC4, deletion of the last 4 c-terminal amino acids | Sorghum bicolor | |
7.2 | - |
phosphoenolpyruvate | G961V | Sorghum bicolor | |
9.5 | - |
phosphoenolpyruvate | DELTAC1, deletion of the last c-terminal amino acid | Sorghum bicolor | |
17.4 | - |
phosphoenolpyruvate | G961A | Sorghum bicolor | |
66 | - |
phosphoenolpyruvate | - |
Sorghum bicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sorghum bicolor |