Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | activates | Starkeya novella | |
butyryl-CoA | activates | Starkeya novella | |
heptanoyl-CoA | activates | Starkeya novella | |
hexanoyl-CoA | activates | Starkeya novella | |
propionyl-CoA | activates | Starkeya novella | |
succinyl-CoA | activates | Starkeya novella | |
valeryl-CoA | activates | Starkeya novella |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Starkeya novella | |
0.11 | - |
HCO3- | - |
Starkeya novella | |
0.64 | - |
phosphoenolpyruvate | - |
Starkeya novella |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 28% of the activation with Mg2+ | Starkeya novella | |
Co2+ | 30% of the activation with Mg2+ | Starkeya novella | |
Mg2+ | divalent cation required, Mg2+ is the best activator | Starkeya novella | |
Mn2+ | 68% of the activation with Mg2+. At suboptimal concentrations of phosphoenolpyruvate and HCO3-, Mn2+ is more effective than Mg2+ | Starkeya novella |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
4 * 95000, SDS-PAGE | Starkeya novella |
360000 | - |
gel filtration | Starkeya novella |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Phosphoenolpyruvate + CO2 | Starkeya novella | function is probably anaplerotic | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Starkeya novella | - |
strain ATCC 90893 | - |
Purification (Comment) | Organism |
---|---|
- |
Starkeya novella |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51.09 | - |
- |
Starkeya novella |
Storage Stability | Organism |
---|---|
-20°C, 0.04 M Tris/HCl buffer, pH 7.8, 2.0 mM dithiothreitol, 2.0 mM MgCl2, 1.0 mM EDTA, 20% glycerol, 1.0 mM Pro, stable for at least 4 weeks | Starkeya novella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + CO2 | - |
Starkeya novella | phosphate + oxaloacetate | - |
? | |
Phosphoenolpyruvate + CO2 | function is probably anaplerotic | Starkeya novella | ? | - |
? | |
phosphoenolpyruvate + HCO3- | - |
Starkeya novella | phosphate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 95000, SDS-PAGE | Starkeya novella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Starkeya novella |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | activates | Starkeya novella |