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Literature summary for 4.1.1.31 extracted from

  • Charles, A.M.; Sykora, Y.
    Purification and characterization of the phosphoenolpyruvate carboxylase from the facultative chemolithotroph Thiobacillus novellus (ATCC 8093) (1992), Antonie van Leeuwenhoek, 62, 155-165.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetoacetyl-CoA activates Starkeya novella
butyryl-CoA activates Starkeya novella
heptanoyl-CoA activates Starkeya novella
hexanoyl-CoA activates Starkeya novella
propionyl-CoA activates Starkeya novella
succinyl-CoA activates Starkeya novella
valeryl-CoA activates Starkeya novella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Starkeya novella
0.11
-
HCO3-
-
Starkeya novella
0.64
-
phosphoenolpyruvate
-
Starkeya novella

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ 28% of the activation with Mg2+ Starkeya novella
Co2+ 30% of the activation with Mg2+ Starkeya novella
Mg2+ divalent cation required, Mg2+ is the best activator Starkeya novella
Mn2+ 68% of the activation with Mg2+. At suboptimal concentrations of phosphoenolpyruvate and HCO3-, Mn2+ is more effective than Mg2+ Starkeya novella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
4 * 95000, SDS-PAGE Starkeya novella
360000
-
gel filtration Starkeya novella

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Phosphoenolpyruvate + CO2 Starkeya novella function is probably anaplerotic ?
-
?

Organism

Organism UniProt Comment Textmining
Starkeya novella
-
strain ATCC 90893
-

Purification (Commentary)

Purification (Comment) Organism
-
Starkeya novella

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
51.09
-
-
Starkeya novella

Storage Stability

Storage Stability Organism
-20°C, 0.04 M Tris/HCl buffer, pH 7.8, 2.0 mM dithiothreitol, 2.0 mM MgCl2, 1.0 mM EDTA, 20% glycerol, 1.0 mM Pro, stable for at least 4 weeks Starkeya novella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
Starkeya novella phosphate + oxaloacetate
-
?
Phosphoenolpyruvate + CO2 function is probably anaplerotic Starkeya novella ?
-
?
phosphoenolpyruvate + HCO3-
-
Starkeya novella phosphate + oxaloacetate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 95000, SDS-PAGE Starkeya novella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Starkeya novella

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA activates Starkeya novella