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Literature summary for 4.1.1.31 extracted from

  • Bramwell, H.; Nimmo, H.G.; Hunter, I.S.; Coggins, J.R.
    Phosphoenolpyruvate carboxylase from Streptomyces coelicolor A3(2): purification of the enzyme, cloning of the ppc gene and over-expression of the protein in a streptomycete (1993), Biochem. J., 293, 131-136.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
subcloned into the streptomycete high-copy-number plasmid vector pIJ486 and transferred into Streptomyces lividans Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces coelicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42.9
-
-
Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
Streptomyces coelicolor phosphate + oxaloacetate
-
?
phosphoenolpyruvate + CO2
-
Streptomyces coelicolor A3(2) phosphate + oxaloacetate
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Streptomyces coelicolor