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Literature summary for 4.1.1.31 extracted from
- Purification and properties of phosphoenolpyruvate carboxylase from immature pods of chickpea (Cicer arietinum L.) (1986), Plant Physiol., 80, 369-373.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-Phosphoglyceric acid | activates | Cicer arietinum |  |
| alpha-glycerophosphate | activates | Cicer arietinum | |
| fructose 1,6-bisphosphate | activates | Cicer arietinum | |
| glucose 6-phosphate | activates | Cicer arietinum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Cicer arietinum | |
| Ca2+ | 5 mM, in presence of 5 mM Mg2+, 83% inhibition | Cicer arietinum | |
| CTP | - |
Cicer arietinum | |
| GTP | - |
Cicer arietinum | |
| malate | - |
Cicer arietinum | |
| Mn2+ | 5 mM, in presence of 5 mM Mg2+, 72% inhibition | Cicer arietinum | |
| oxaloacetate | - |
Cicer arietinum | |
| UTP | - |
Cicer arietinum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.74 | - |
phosphoenolpyruvate | - |
Cicer arietinum | |
| 7.6 | - |
HCO3- | - |
Cicer arietinum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Cicer arietinum | |
| Mg2+ | Km: 0.56 mM | Cicer arietinum | |
| Mn2+ | no activation | Cicer arietinum | |
| phosphate | activates | Cicer arietinum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
4 * 50000, SDS-PAGE | Cicer arietinum |
| 200000 | - |
gel filtration | Cicer arietinum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cicer arietinum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cicer arietinum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pod | immature | Cicer arietinum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.137 | - |
- |
Cicer arietinum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, stable for 3 d | Cicer arietinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + CO2 | - |
Cicer arietinum | phosphate + oxaloacetate | - |
? | |
| phosphoenolpyruvate + HCO3- | - |
Cicer arietinum | phosphate + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 50000, SDS-PAGE | Cicer arietinum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
2 min, 56% loss of activity | Cicer arietinum |
| 60 | - |
2 min, complete loss of activity | Cicer arietinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
- |
Cicer arietinum |
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Release 2023.1 (January 2023)
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