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Literature summary for 4.1.1.31 extracted from
- Phosphoenolpyruvate carboxylase from Acetobacter aceti (1979), Arch. Microbiol., 122, 109-115.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| propionyl-CoA | absolute requirement for acetyl-CoA or propionyl-CoA | Acetobacter aceti |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Acetobacter aceti | |
| Asp | - |
Acetobacter aceti | |
| citrate | - |
Acetobacter aceti | |
| Co2+ | maximal activity at 1 mM Co2+, strong decrease of activity above 4 mM | Acetobacter aceti | |
| fumarate | - |
Acetobacter aceti | |
| Glu | - |
Acetobacter aceti | |
| Mn2+ | maximal activity at 0.5 mM Mn2+, strong decrease of activity above 2 mM | Acetobacter aceti | |
| p-hydroxymercuribenzoate | glutathione protects | Acetobacter aceti | |
| PCMB | glutathione protects | Acetobacter aceti | |
| succinate | - |
Acetobacter aceti | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
phosphoenolpyruvate | - |
Acetobacter aceti | |
| 3 | - |
HCO3- | - |
Acetobacter aceti | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation required, Mn2+, Co2+ and Mg2+. Co2+ is less effective than Mg2+ or Mn2+ | Acetobacter aceti | |
| Co2+ | maximal activity at 1 mM Co2+, strong decrease of activity above 4 mM | Acetobacter aceti | |
| Co2+ | Km: 0.26 mM | Acetobacter aceti | |
| Mg2+ | Km: 0.77 mM | Acetobacter aceti | |
| Mg2+ | divalent cation required, Mn2+, Co2+ and Mg2+ | Acetobacter aceti | |
| Mn2+ | divalent cation required, Mn2+, Co2+ and Mg2+ | Acetobacter aceti | |
| Mn2+ | Km: 0.12 mM | Acetobacter aceti | |
| Mn2+ | maximal activity at 0.5 mM Mn2+, strong decrease of activity above 2 mM | Acetobacter aceti | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 380000 | 400000 | gel filtration | Acetobacter aceti |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacter aceti | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetobacter aceti |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.9 | - |
- |
Acetobacter aceti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + CO2 | - |
Acetobacter aceti | phosphate + oxaloacetate | - |
? | |
| phosphoenolpyruvate + HCO3- | - |
Acetobacter aceti | phosphate + oxaloacetate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Acetobacter aceti |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | rapid inactivation | Acetobacter aceti |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Acetobacter aceti |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | about 80% of maximal activity at pH 6.5 and at pH 9.0 | Acetobacter aceti |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | absolute requirement for acetyl-CoA or propionyl-CoA | Acetobacter aceti | |
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