Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.29 extracted from

  • Deng, J.; Wu, Q.; Gao, H.; Ou, Q.; Wu, B.; Yan, B.; Jiang, C.
    Molecular characterization and directed evolution of a metagenome-derived L-cysteine sulfinate decarboxylase (2018), Food Technol. Biotechnol., 56, 117-123 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.29

Cloned(Commentary)

Cloned (Comment) Organism
gene undec1A, cloned from metagenome through sequence-based screening of uncultured soil microorganisms, DNA and amino acid sequence determination and analysis, recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS unidentified prokaryotic organism

Protein Variants

Protein Variants Comment Organism
V81L/F240S/I250S/D266L generation of mutant Undec1A-1180 by random mutagenxadesis through sequential error-prone PCR from a mutagenesis library. The mutant has 5.62fold highxader specific activity than wild-type Undec1A at 35°C and pH 7.0. The optimum pH of Undec1A-1180 does not change. Molecular docking results indicatxade that amino acid residues Ala235, Val237, Asp239, Ile267, Ala268, and Lys298 in mutant Unxaddec1A-1180 protein help recognize substrate molecules and catalyze the reaction with of L-cysteine sulfinic acid. Mutation efficiency analysis of different combinations of Mg2+ and Mn2+, overview unidentified prokaryotic organism

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Lineweaver-Burk plots unidentified prokaryotic organism
1.1
-
 3-sulfino-L-alanine pH 7.0, 35°C, recombinant mutant V81L/F240S/I250S/D266L enzyme unidentified prokaryotic organism
1.56
-
 3-sulfino-L-alanine pH 7.0, 35°C, recombinant wild-type enzyme unidentified prokaryotic organism

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-sulfino-L-alanine unidentified prokaryotic organism
-
 hypotaurine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
unidentified prokaryotic organism A0A2I9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography unidentified prokaryotic organism

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-sulfino-L-alanine
-
 unidentified prokaryotic organism hypotaurine + CO2
-
 ?
additional information substrate specificity, overview. Substrate analogues L-cysteine, L-proline, L-alanine, L-glutamate, and L-asparagxadinate (at 5 mM) are also used as substrates by enzyme mutant Undec1A-1180, highest activity with L-cysteine, low activity with L-proline, no activity with L-alanine unidentified prokaryotic organism ?
-
 ?

Synonyms

Synonyms Comment Organism
CSD
-
 unidentified prokaryotic organism
L-cysteine sulfinate decarboxylase
-
 unidentified prokaryotic organism
undec1A
-
 unidentified prokaryotic organism

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 recombinant His-tagged wild-type and mutant enzymes unidentified prokaryotic organism

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.763
-
 3-sulfino-L-alanine pH 7.0, 35°C, recombinant wild-type enzyme unidentified prokaryotic organism
1.476
-
 3-sulfino-L-alanine pH 7.0, 35°C, recombinant mutant V81L/F240S/I250S/D266L enzyme unidentified prokaryotic organism

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant His-tagged wild-type and mutant enzymes unidentified prokaryotic organism

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 unidentified prokaryotic organism

General Information

General Information Comment Organism
malfunction the mutant Undec1A-1180 has 5.62fold highxader specific activity than wild-type Undec1A unidentified prokaryotic organism
additional information structure homology modelling and molecular docking, overview unidentified prokaryotic organism
physiological function CSD is the rate-limiting enzyme of taurine biosynthesis unidentified prokaryotic organism

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.49
-
 3-sulfino-L-alanine pH 7.0, 35°C, recombinant wild-type enz.yme unidentified prokaryotic organism
1.34
-
 3-sulfino-L-alanine pH 7.0, 35°C, recombinant mutant V81L/F240S/I250S/D266L enzyme unidentified prokaryotic organism