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Literature summary for 4.1.1.28 extracted from

  • Nakazawa, H.; Kumagai, H.; Yamada, H.
    Aromatic L-amino acid decarboxylase from Micrococcus percitreus purification, crystallization and properties (1981), Agric. Biol. Chem., 45, 2543-2552 .
No PubMed abstract available
Search for more literature for 4.1.1.28

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 L-tryptophan at pH 9.0 and 30°C Micrococcus percitreus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
101000
-
 gel filtration Micrococcus percitreus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan Micrococcus percitreus 100% activity tryptamine + CO2
-
 ?
L-tryptophan Micrococcus percitreus AJ 1065 100% activity tryptamine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Micrococcus percitreus
-
-
-
Micrococcus percitreus AJ 1065
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sephadex column chromatography, and Sephadex G-200 gel filtration Micrococcus percitreus

Storage Stability

Storage Stability Organism
5°C, 0.5 M potassium phosphate buffer pH 7.0 containing 20% (v/v) glycerol and 10 mM pyridoxal phosphate, more than 10 months, no loss of activity Micrococcus percitreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-L-tyrosine 77% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
2-methyl-L-tyrosine 75% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
3,4-Dihydroxy-L-phenylalanine 10% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
3,4-Dihydroxy-L-phenylalanine 10% activity compared to L-tryptophan Micrococcus percitreus AJ 1065 ?
-
 ?
3,5-dibromo-DL-tyrosine 72% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
3-chloro-L-tyrosine 81% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
3-Hydroxy-DL-kynurenine 8% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
3-Hydroxy-DL-kynurenine 8% activity compared to L-tryptophan Micrococcus percitreus AJ 1065 ?
-
 ?
3-methyl-L-tyrosine 80% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
5-fluoro-DL-tryptophan 75% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
5-hydroxy-L-tryptophan 24% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
5-methyl-DL-tryptophan 17% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
7-aza-DL-tryptophan 8% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
alpha-methyl-DL-phenylalanine 2% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
alpha-methyl-DL-tyrosine 1% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
L-2,4-dihydroxyphenylalanine 75% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
L-kynurenine 15% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
L-phenylalanine 120% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
L-tryptophan 100% activity Micrococcus percitreus tryptamine + CO2
-
 ?
L-tryptophan 100% activity Micrococcus percitreus AJ 1065 tryptamine + CO2
-
 ?
L-tyrosine 87% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
L-tyrosine 87% activity compared to L-tryptophan Micrococcus percitreus AJ 1065 ?
-
 ?
m-DL-tyrosine 5% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
additional information no activity with 4-nitro-DL-phenylalanine, 3,5-dinitro-DL-tyrosine, 2,4,4-trihydroxyphenyl-L-alanine, alpha-methyl-3,4-dihydroxy-phenyl-L-alanine, 2-phenyl-DL-serine, 2-thiazole-DL-alanine, and L-histidine Micrococcus percitreus ?
-
 ?
additional information no activity with 4-nitro-DL-phenylalanine, 3,5-dinitro-DL-tyrosine, 2,4,4-trihydroxyphenyl-L-alanine, alpha-methyl-3,4-dihydroxy-phenyl-L-alanine, 2-phenyl-DL-serine, 2-thiazole-DL-alanine, and L-histidine Micrococcus percitreus AJ 1065 ?
-
 ?
o-DL-tyrosine 32% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 48000, SDS-PAGE Micrococcus percitreus

Synonyms

Synonyms Comment Organism
Aromatic L-amino acid decarboxylase
-
 Micrococcus percitreus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 50 the purified enzyme is stable until 35°C for 60 min but rapidly inactivated at above 50°C Micrococcus percitreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Micrococcus percitreus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 the enzyme is stable for 60 min between pH 6.0 and 7.0 Micrococcus percitreus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Micrococcus percitreus