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Literature summary for 4.1.1.28 extracted from

  • Bertoldi, M.; Voltattorni, C.B.
    Multiple roles of the active site lysine of Dopa decarboxylase (2009), Arch. Biochem. Biophys., 488, 130-139.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzyme K303A is expressed in Escherichia coli SVS370 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K303A the mutant binds pyridoxal 5'-phosphate with a 100fold decreased apparent equilibrium binding affinity with respect to the wild type enzyme. Unlike the wild-type, K303A in the presence of L-Dopa displays a parallel progress course of formation of both dopamine and 3,4-dihydroxyphenylacetaldehyde (plus ammonia) with a burst followed by a linear phase Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
L-Dopa slight substrate inhibition is observed at high concentration of L-Dopa Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000095
-
L-Dopa dopamine formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
0.00083
-
dopamine mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
0.00092
-
L-Dopa aldehyde formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
0.0505
-
dopamine wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
4.27
-
L-Dopa wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Discovery C18 column chromatography, gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Dopa
-
Homo sapiens dopamine + CO2
-
?

Synonyms

Synonyms Comment Organism
DDC
-
Homo sapiens
DOPA decarboxylase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
L-Dopa wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
0.11
-
L-Dopa aldehyde formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
0.188
-
L-Dopa dopamine formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
0.7
-
dopamine mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens
2.48
-
dopamine wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Homo sapiens