Cloned (Comment) | Organism |
---|---|
mutant enzyme K303A is expressed in Escherichia coli SVS370 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K303A | the mutant binds pyridoxal 5'-phosphate with a 100fold decreased apparent equilibrium binding affinity with respect to the wild type enzyme. Unlike the wild-type, K303A in the presence of L-Dopa displays a parallel progress course of formation of both dopamine and 3,4-dihydroxyphenylacetaldehyde (plus ammonia) with a burst followed by a linear phase | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Dopa | slight substrate inhibition is observed at high concentration of L-Dopa | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000095 | - |
L-Dopa | dopamine formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C | Homo sapiens | |
0.00083 | - |
dopamine | mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C | Homo sapiens | |
0.00092 | - |
L-Dopa | aldehyde formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C | Homo sapiens | |
0.0505 | - |
dopamine | wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C | Homo sapiens | |
4.27 | - |
L-Dopa | wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Discovery C18 column chromatography, gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Dopa | - |
Homo sapiens | dopamine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DDC | - |
Homo sapiens |
DOPA decarboxylase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
L-Dopa | wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C | Homo sapiens | |
0.11 | - |
L-Dopa | aldehyde formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C | Homo sapiens | |
0.188 | - |
L-Dopa | dopamine formation, mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C | Homo sapiens | |
0.7 | - |
dopamine | mutant enzyme K303A, in 50 mM HEPES, pH 7.5, at 25°C | Homo sapiens | |
2.48 | - |
dopamine | wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |