Any feedback?
Literature summary for 4.1.1.28 extracted from
- Expression of enzymatically active L-DOPA decarboxylase in human peripheral leukocytes (2008), Blood Cells Mol. Dis., 42, 92-98.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
| membrane | a population of DDC molecules are intrinsic membrane proteins | Homo sapiens | 16020 | - |
| additional information | a significant number of proteins exist in membrane-associated and soluble forms | Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
alternative DDC isoform, SDS-PAGE | Homo sapiens |
| 37000 | - |
determined by SDS-PAGE, determined by SDS-PAGE, expression of the alternative DDC molecule (alt-DDC) in human peripheral leukocytes, lacks exons 10-15 but includes an alternative exon 10 | Homo sapiens |
| 54000 | - |
determined by SDS-PAGE, expression of DDC in human T-lymphoytes | Homo sapiens |
| 54000 | - |
native DDC isoform, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Dopa | Homo sapiens | - |
dopamine + CO2 | - |
? |  |
| additional information | Homo sapiens | regulating factor of the proliferation and differentiation of different leukocyte subtypes | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| connective tissue | neural and non-neural type DDC | Homo sapiens | - |
| leukocyte | peripheral | Homo sapiens | - |
| leukocyte | peripheral leukocyte | Homo sapiens | - |
| additional information | tissue specificity is proposed to be directed by both alternative promoter usage and alternative splicing | Homo sapiens | - |
| T-lymphocyte | - |
Homo sapiens | - |
| white blood cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
although the obtained specific activity values are found to be lower than the activity values observed in embryonic K293 cells, the data strongly suggest the endogenous dopamine production in these types of cells and underline the complexity of the dopamine synthetic pathway | Homo sapiens |
| additional information | - |
the purified enzyme shows a specific activity of 3.67 units/mg at 37°C | Homo sapiens |
| 3.67 | - |
activity in peripheral leukocytes, determined by radiochemical method at 37°C | Homo sapiens |
| 10 | - |
positive controls (embryonic K293 cells) | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Dopa | - |
Homo sapiens | dopamine + CO2 | - |
? | |
| additional information | regulating factor of the proliferation and differentiation of different leukocyte subtypes | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | homodimer binding one pyridoxal 5'-phosphate molecule | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AADC | - |
Homo sapiens |
| Aromatic L-amino acid decarboxylase | - |
Homo sapiens |
| DDC | - |
Homo sapiens |
| DDC | human DDC gene undergoes complex processing leading to the formation of multiple mRNA isoforms. Alternative splicing within its 5'-untranslated region leads to the formation of two mRNA types, neural (N1 exon) and non-neural (L1 exon) type. Neural type DDC transcript which lacks exon 3 and the alternative 37 kD alt-DDC protein isoform which lacks exons 10-15 but includes an alternative exon 10 in human peripheral leukocytes. | Homo sapiens |
| L-3,4-Dihydroxyphenylalanine decarboxylase | - |
Homo sapiens |
| L-DOPA decarboxylase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
