Crystallization (Comment) | Organism |
---|---|
- |
Micrococcus percitreus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.98 | - |
L-Phe | - |
Micrococcus percitreus | |
1.1 | - |
L-Tyr | - |
Micrococcus percitreus | |
2.3 | - |
3,4-dihydroxyphenylalanine | - |
Micrococcus percitreus | |
2.4 | - |
L-Trp | - |
Micrococcus percitreus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, SDS-PAGE | Micrococcus percitreus |
101000 | - |
gel filtration | Micrococcus percitreus |
105000 | - |
equilibrium sedimentation | Micrococcus percitreus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus percitreus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Micrococcus percitreus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.7 | - |
- |
Micrococcus percitreus |
Storage Stability | Organism |
---|---|
5°C, in 0.5 M potassium phosphate buffer, pH 7.0, 20% glycerol, 1 mM pyridoxal phosphate and 19 mM 2-mercaptoethanol, stable for more than 10 months | Micrococcus percitreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-Dibromo-DL-Tyr | 75% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? | |
2-Chloro-L-Tyr | 77% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? | |
2-Methyl-L-Tyr | 75% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? | |
3,4-Dibromo-DL-Tyr | 10% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? | |
3,4-Dihydroxyphenylalanine | - |
Micrococcus percitreus | 3-Hydroxytyramine + CO2 | - |
? | |
3,5-Dibromo-DL-Tyr | 72% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? | |
3-Chloro-L-Tyr | 81% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? | |
3-Hydroxy-DL-kynurenine | 8% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? | |
3-Methyl-L-Tyr | 80% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? | |
5-Fluoro-DL-Trp | 75% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? | |
5-Hydroxytryptophan | 24% of the activity with L-Trp | Micrococcus percitreus | Serotonin + CO2 | - |
? | |
5-Methyl-DL-Trp | 17% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? | |
7-Aza-DL-Trp | 8% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? | |
L-kynurenine | 15% of the activity with L-Trp | Micrococcus percitreus | 3-amino-1-(2-aminophenyl)propan-1-one + CO2 | - |
? | |
L-Phe | m-DL-Tyr, 17% of the activity with L-Trp | Micrococcus percitreus | phenylethylamine + CO2 | - |
? | |
L-Phe | o-DL-Tyr, 17% of the activity with L-Trp | Micrococcus percitreus | phenylethylamine + CO2 | - |
? | |
L-Phe | L-Tyr, 120% of the activity with L-Trp | Micrococcus percitreus | phenylethylamine + CO2 | - |
? | |
L-Trp | - |
Micrococcus percitreus | Tryptamine + CO2 | - |
? | |
Tyr | 87% of the activity with L-Trp | Micrococcus percitreus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, SDS-PAGE | Micrococcus percitreus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
in the presence of high concentrations of pyridoxal phosphate, glycerol or potassium phosphate buffer, pH 7.0, stability against heating increases | Micrococcus percitreus |
30 | - |
60 min, stable between pH 6.0 and 7.0 | Micrococcus percitreus |
35 | - |
pH 7.0, 5 min, stable until | Micrococcus percitreus |
50 | - |
pH 7.0, 5 min, rapid inactivation above | Micrococcus percitreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Micrococcus percitreus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
30°C, 60 min, about 70% loss of activity | Micrococcus percitreus |
5 | - |
30°C, 60 min, about 35% loss of activity | Micrococcus percitreus |
6 | 7 | 30°C, 60 min, stable | Micrococcus percitreus |
8 | - |
30°C, 60 min, about 20% loss of activity | Micrococcus percitreus |
10 | - |
30°C, 60 min, about 40% loss of activity | Micrococcus percitreus |
11 | - |
30°C, 60 min, about 85% loss of activity | Micrococcus percitreus |