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Literature summary for 4.1.1.28 extracted from

  • Nakazawa, H.; Kumagai, H.; Yamada, H.
    Aromatic L-amino acid decarboxylase from Micrococcus percitreus. Purification, crystallization and properties (1981), Agric. Biol. Chem., 45, 2543-2552.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Micrococcus percitreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.98
-
L-Phe
-
Micrococcus percitreus
1.1
-
L-Tyr
-
Micrococcus percitreus
2.3
-
3,4-dihydroxyphenylalanine
-
Micrococcus percitreus
2.4
-
L-Trp
-
Micrococcus percitreus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
2 * 48000, SDS-PAGE Micrococcus percitreus
101000
-
gel filtration Micrococcus percitreus
105000
-
equilibrium sedimentation Micrococcus percitreus

Organism

Organism UniProt Comment Textmining
Micrococcus percitreus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Micrococcus percitreus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37.7
-
-
Micrococcus percitreus

Storage Stability

Storage Stability Organism
5°C, in 0.5 M potassium phosphate buffer, pH 7.0, 20% glycerol, 1 mM pyridoxal phosphate and 19 mM 2-mercaptoethanol, stable for more than 10 months Micrococcus percitreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-Dibromo-DL-Tyr 75% of the activity with L-Trp Micrococcus percitreus ?
-
?
2-Chloro-L-Tyr 77% of the activity with L-Trp Micrococcus percitreus ?
-
?
2-Methyl-L-Tyr 75% of the activity with L-Trp Micrococcus percitreus ?
-
?
3,4-Dibromo-DL-Tyr 10% of the activity with L-Trp Micrococcus percitreus ?
-
?
3,4-Dihydroxyphenylalanine
-
Micrococcus percitreus 3-Hydroxytyramine + CO2
-
?
3,5-Dibromo-DL-Tyr 72% of the activity with L-Trp Micrococcus percitreus ?
-
?
3-Chloro-L-Tyr 81% of the activity with L-Trp Micrococcus percitreus ?
-
?
3-Hydroxy-DL-kynurenine 8% of the activity with L-Trp Micrococcus percitreus ?
-
?
3-Methyl-L-Tyr 80% of the activity with L-Trp Micrococcus percitreus ?
-
?
5-Fluoro-DL-Trp 75% of the activity with L-Trp Micrococcus percitreus ?
-
?
5-Hydroxytryptophan 24% of the activity with L-Trp Micrococcus percitreus Serotonin + CO2
-
?
5-Methyl-DL-Trp 17% of the activity with L-Trp Micrococcus percitreus ?
-
?
7-Aza-DL-Trp 8% of the activity with L-Trp Micrococcus percitreus ?
-
?
L-kynurenine 15% of the activity with L-Trp Micrococcus percitreus 3-amino-1-(2-aminophenyl)propan-1-one + CO2
-
?
L-Phe m-DL-Tyr, 17% of the activity with L-Trp Micrococcus percitreus phenylethylamine + CO2
-
?
L-Phe o-DL-Tyr, 17% of the activity with L-Trp Micrococcus percitreus phenylethylamine + CO2
-
?
L-Phe L-Tyr, 120% of the activity with L-Trp Micrococcus percitreus phenylethylamine + CO2
-
?
L-Trp
-
Micrococcus percitreus Tryptamine + CO2
-
?
Tyr 87% of the activity with L-Trp Micrococcus percitreus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Micrococcus percitreus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
in the presence of high concentrations of pyridoxal phosphate, glycerol or potassium phosphate buffer, pH 7.0, stability against heating increases Micrococcus percitreus
30
-
60 min, stable between pH 6.0 and 7.0 Micrococcus percitreus
35
-
pH 7.0, 5 min, stable until Micrococcus percitreus
50
-
pH 7.0, 5 min, rapid inactivation above Micrococcus percitreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Micrococcus percitreus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
30°C, 60 min, about 70% loss of activity Micrococcus percitreus
5
-
30°C, 60 min, about 35% loss of activity Micrococcus percitreus
6 7 30°C, 60 min, stable Micrococcus percitreus
8
-
30°C, 60 min, about 20% loss of activity Micrococcus percitreus
10
-
30°C, 60 min, about 40% loss of activity Micrococcus percitreus
11
-
30°C, 60 min, about 85% loss of activity Micrococcus percitreus