Protein Variants | Comment | Organism |
---|---|---|
Q215A | mutant, effect of the mutation of the kinetic parameters for decarboxylation is determined | Saccharomyces cerevisiae |
S154A | mutant, effect of the mutation of the kinetic parameters for decarboxylation is determined | Saccharomyces cerevisiae |
S154A/Q215A | mutant, effect of the mutation of the kinetic parameters for decarboxylation is determined | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
orotidine 5'-monophosphate | wild type enzyme | Saccharomyces cerevisiae | |
0.05 | - |
orotidine 5'-monophosphate | Q215A mutant | Saccharomyces cerevisiae | |
0.11 | - |
orotidine 5'-monophosphate | S154/Q215A mutant | Saccharomyces cerevisiae | |
0.13 | - |
orotidine 5'-monophosphate | S154 mutant | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Saccharomyces cerevisiae | - |
UMP + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P03962 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | - |
Saccharomyces cerevisiae | UMP + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Orotidine 5'-monophosphate decarboxylase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
orotidine 5'-monophosphate | S154/Q215A mutant | Saccharomyces cerevisiae | |
0.082 | - |
orotidine 5'-monophosphate | S154 mutant | Saccharomyces cerevisiae | |
15 | - |
orotidine 5'-monophosphate | wild type enzyme | Saccharomyces cerevisiae | |
21 | - |
orotidine 5'-monophosphate | Q215A mutant | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
activity assay | Saccharomyces cerevisiae |