Application | Comment | Organism |
---|---|---|
medicine | orotidine 5-monophosphate decarboxylase of Plasmodium falciparum could be a target for the development of antimalarial drugs | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
functional enzyme expression in Escherichia coli | Plasmodium falciparum |
into the vector pTrcHis-TOPO for expression in Escherichia coli TOP10 cells | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
apo, substrate or product-complex forms of OMPDC, X-ray diffraction structure determination and anaylsis at 2.65-2.7 A | Plasmodium falciparum |
the crystal structures of the apo form of OMPDC, and in complex with OMP and UMP are resolved at resolutions of 2.7, 2.65 and 2.65 A, respectively | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
monomer, determined by SDS-PAGE | Plasmodium falciparum |
76000 | - |
active recombinant enzyme, gel filtration | Plasmodium falciparum |
76000 | - |
homodimer | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Plasmodium falciparum | - |
UMP + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
functional recombinant enzyme 300fold from Escherichia coli by nickel affinity chromatography and gel filtration | Plasmodium falciparum |
using a Ni2+-nitrilotriacetic acid-agarose affinity and a Superdex-75 column | Plasmodium falciparum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
orotidine 5'-phosphate = UMP + CO2 | decarboxylation mechanism, substrate recognition mechanism with dynamic structural changes, as well as the rearrangement of the hydrogen bond array at the active site | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | - |
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | - |
Plasmodium falciparum | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | change in active-site structure upon binding of orotidine 5'-phosphate/UMP, the backbone amide of Arg294 interacts directly with the phosphate group of the ligands, rearrangement of hydrogen-bond network around Lys102, overview | Plasmodium falciparum | UMP + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, active recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
homodimer | - |
Plasmodium falciparum |
More | structure-activity analysis, overview | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
OMP decarboxylase | - |
Plasmodium falciparum |
OMPDC | - |
Plasmodium falciparum |
orotidine 5-monophosphate decarboxylase | - |
Plasmodium falciparum |
PfOMPDC | - |
Plasmodium falciparum |