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Literature summary for 4.1.1.23 extracted from

  • Callahan, B.P.; Miller, B.G.
    OMP decarboxylase - An enigma persists (2007), Bioorg. Chem., 35, 465-469.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D91A inactive mutant Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
6-hydroxyUMP a transition state analogue inhibitor, binding structure in complex with the enzyme, overview Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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additional information kinetics and thermodynamics Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Orotidine 5'-phosphate Saccharomyces cerevisiae the enzyme is the most effective pure protein catalyst known in nature UMP + CO2
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Reaction

Reaction Comment Organism Reaction ID
orotidine 5'-phosphate = UMP + CO2 reaction mechanism involving the active site aspartate residue Asp91, electrostatic stress furnishes the majority of the catalytic driving force for OMP decarboxylation, ylide generation is not the rate-limiting step in the ODCase reaction Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the yeast enzyme does not exhibit a measurable affinity for a substrate analogue in which the labile carboxylate group is replaced by a cationic substituent, the apo and ligand-bound forms of the enzyme show distinct open and closed forms, the closed form is catalytically competent Saccharomyces cerevisiae ?
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Orotidine 5'-phosphate the enzyme is the most effective pure protein catalyst known in nature Saccharomyces cerevisiae UMP + CO2
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?
Orotidine 5'-phosphate the decarboxylase shows an extremely fast rate acceleration, 1017-fold rate enhancement, ODCase associates weakly with the substrate in the ground state, but then tightens its grip as the altered substrate approaches the chemical transition state, yielding a complex with an estimated dissociation constant Ktx of over 10-24 M Saccharomyces cerevisiae UMP + CO2
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?

Synonyms

Synonyms Comment Organism
ODCase
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Saccharomyces cerevisiae
OMP decarboxylase
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Saccharomyces cerevisiae