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Literature summary for 4.1.1.23 extracted from

  • Miller, B.G.; Snider, M.J.; Wolfenden, R.; Short, S.A.
    Dissecting a charged network at the active site of orotidine-5'-phosphate decarboxylase (2001), J. Biol. Chem., 276, 15174-15176.
    View publication on PubMed
Search for more literature for 4.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant ODCase, expression in Escherichia coli SS6130 Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with 6-hydroxyuridine 5’-phosphate Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D91A inactive mutant, reduced activity by 5 orders of magnitude, no substrate binding Saccharomyces cerevisiae
D96A inactive mutant, reduced kcat-value by more than 5 orders of magnitude, 11fold decrease in the affinity for the substrate in the ground state Saccharomyces cerevisiae
K59A 130fold reduced kcat-value, 900fold increased Km-value Saccharomyces cerevisiae
K93A inactive mutant, reduced activity by 5 orders of magnitude, no substrate binding Saccharomyces cerevisiae
Q215A same activity as wild-type enzyme Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
6-azauridine 5'-phosphate
-
 Saccharomyces cerevisiae
6-hydroxyuridine 5'-phosphate
-
 Saccharomyces cerevisiae
UMP
-
 Saccharomyces cerevisiae
xanthosine 5'-phosphate
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Saccharomyces cerevisiae
0.0007
-
 orotidine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae
0.008
-
 2'-deoxyorotidine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae
0.025
-
 orotidine 5'-phosphate Q215A mutant Saccharomyces cerevisiae
0.64
-
 orotidine 5'-phosphate K59A mutant Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal cofactor requirement Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Orotidine 5'-phosphate Saccharomyces cerevisiae
-
 UMP + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzyme Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
orotidine 5'-phosphate = UMP + CO2 mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00018
-
 below, K93A mutant Saccharomyces cerevisiae
0.00047
-
 below, D96A muatnt Saccharomyces cerevisiae
0.00066
-
 below, D91A mutant Saccharomyces cerevisiae
0.19
-
 K59A mutant Saccharomyces cerevisiae
84
-
 Q215A mutant Saccharomyces cerevisiae
90
-
 wild-type enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyorotidine 5'-phosphate
-
 Saccharomyces cerevisiae 2'-deoxyuridine 5'-phosphate + CO2
-
 ?
Orotidine 5'-phosphate
-
 Saccharomyces cerevisiae UMP + CO2
-
 ?
Orotidine 5'-phosphate the integrity of the network of the charged residues within the active site, Lys-59, Asp-91, Lys-93 and Asp-96, is essential for transition state stabilization, altered substrate is very tightly bound in the transition state, mechanism Saccharomyces cerevisiae UMP + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00023
-
 orotidine 5'-phosphate below, D96A mutant Saccharomyces cerevisiae
0.34
-
 orotidine 5'-phosphate K59A mutant Saccharomyces cerevisiae
41
-
 orotidine 5'-phosphate Q215A mutant Saccharomyces cerevisiae
44
-
 orotidine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor requirement Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000000008
-
 6-hydroxyuridine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae
0.000029
-
 6-azauridine 5'-phosphate Q215A mutant Saccharomyces cerevisiae
0.000036
-
 6-azauridine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae
0.000094
-
 6-hydroxyuridine 5'-phosphate K59A mutant Saccharomyces cerevisiae
0.00041
-
 xanthosine 5'-phosphate wild-type enzyme Saccharomyces cerevisiae
0.00046
-
 xanthosine 5'-phosphate Q215A mutant Saccharomyces cerevisiae
0.039
-
 6-azauridine 5'-phosphate K59A mutant Saccharomyces cerevisiae
0.17
-
 UMP Q215A mutant Saccharomyces cerevisiae
0.2
-
 UMP wild-type enzyme Saccharomyces cerevisiae
0.33
-
 UMP K59A mutant Saccharomyces cerevisiae
0.73
-
 xanthosine 5'-phosphate K59A mutant Saccharomyces cerevisiae