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Literature summary for 4.1.1.23 extracted from

  • Miller, B.G.; Smiley, J.A.; Short, S.A.; Wolfenden, R.
    Activity of yeast orotidine-5'-phosphate decarboxylase in the absence of metals (1999), J. Biol. Chem., 274, 23841-23843.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
ura3 gene expression in Escherichia coli SS6130, expression in yeast Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
6-thiocarboxamidouridine 5'-phosphate competitive, very strong, reversible inhibition Saccharomyces cerevisiae
additional information not inhibited by 6-carboxamidouridine 5’-phosphate, recombinant ODCase expressed in Escherichia coli is not inhibited by 1,3-dimercaptopropanol and EDTA Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
orotidine 5'-phosphate 22-25°C, pH 7.2, recombinant ODCase expressed in Escherichia coli Saccharomyces cerevisiae
0.0007
-
orotidine 5'-phosphate 22°C, native ODCase expressed in yeast Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information zinc-free fully active recombinant ODCase expressed in Escherichia coli, metals do not directly participate in the decarboxylation mechanism Saccharomyces cerevisiae
Zn2+ native ODCase expressed in yeast contains zinc, but not recombinant ODCase expressed in Escherichia coli, zinc is not involved in substrate decarboxylation Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 27000, about, recombinant ODCase expressed in Escherichia coli, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Orotidine 5'-phosphate Saccharomyces cerevisiae production of UMP, the biosynthetic precursor of pyrimidine nucleotides UMP + CO2
-
?
Orotidine 5'-phosphate Saccharomyces cerevisiae BJ5424 production of UMP, the biosynthetic precursor of pyrimidine nucleotides UMP + CO2
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BJ5424
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no posttranslational modifications in recombinant ODCase expressed in Escherichia coli, but presumably in native ODCase from yeast, in which the N-terminus is blocked Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
recombinant ODCase expressed in Escherichia coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.2
-
22°C, native ODCase expressed in yeast Saccharomyces cerevisiae
39.3
-
22-25°C, pH 7.2, recombinant ODCase expressed in Escherichia coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Orotidine 5'-phosphate
-
Saccharomyces cerevisiae UMP + CO2
-
?
Orotidine 5'-phosphate production of UMP, the biosynthetic precursor of pyrimidine nucleotides Saccharomyces cerevisiae UMP + CO2
-
?
Orotidine 5'-phosphate
-
Saccharomyces cerevisiae BJ5424 UMP + CO2
-
?
Orotidine 5'-phosphate production of UMP, the biosynthetic precursor of pyrimidine nucleotides Saccharomyces cerevisiae BJ5424 UMP + CO2
-
?

Subunits

Subunits Comment Organism
? x * 27000, about, recombinant ODCase expressed in Escherichia coli, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the recombinant zinc-free ODCase expressed in Escherichia coli is more thermolabile than the zinc-containing ODCase expressed in yeast Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13
-
orotidine 5'-phosphate 22°C, native ODCase expressed in yeast Saccharomyces cerevisiae
19
-
orotidine 5'-phosphate 22-25°C, pH 7.2, recombinant ODCase expressed in Escherichia coli Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000035
-
6-thiocarboxamidouridine 5'-phosphate recombinant ODCase expressed in Escherichia coli Saccharomyces cerevisiae