Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli NF 1830, expression of selenomethionine-substituted ODCase in Escherichia coli SO 6733 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
complexed with the inhibitor 1-(5-phospho-beta-D-ribofuranosyl)barbituric acid | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(5'-phospho-beta-D-ribofuranosyl)barbituric acid | mode of binding | Escherichia coli | |
UMP | weak inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
orotidine 5'-phosphate | pH 7, increases to either side of pH 7 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Escherichia coli | catalyzes the last step in the de novo synthesis of UMP | UMP + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
orotidine 5'-phosphate = UMP + CO2 | catalytic mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
same specific activity of native and selenomethionine-substituted ODCase | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Thioorotidine 5'-phosphate | partially active substrate | Escherichia coli | 4-Thiouridine 5'-phosphate + CO2 | - |
? | |
Orotidine 5'-phosphate | catalytic mechanism, role of Lys-93 in catalysis, enzyme structure | Escherichia coli | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | catalyzes the last step in the de novo synthesis of UMP | Escherichia coli | UMP + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | dimer is composed of two alpha/beta-barrels with two shared active sites | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | very proficient enzyme | Escherichia coli | |
2 | 8 | orotidine 5'-phosphate | pH 7, constant above pH 7, declines below pH 7 | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
very sharp | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor requirement | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
1-(5'-phospho-beta-D-ribofuranosyl)barbituric acid | below | Escherichia coli | |
0.5 | - |
UMP | - |
Escherichia coli |