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Literature summary for 4.1.1.23 extracted from

  • Bell, J.B.; Jones, M.E.
    Purification and characterization of yeast orotidine 5'-monophosphate decarboxylase overexpressed from plasmid PGU2 (1991), J. Biol. Chem., 266, 12662-12667.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-(5'-phospho-beta-D-ribofuranosyl)barbituric acid
-
Saccharomyces cerevisiae
6-azauridine 5'-phosphate
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
orotidine 5'-phosphate pH 6, 25°C Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29500
-
sucrose density gradient centrifugation, at low protein concentration, in absence of ligands that bind at the catalytic site. The enzyme exists as dimer at high protein concentrations Saccharomyces cerevisiae
64000
-
sucrose density gradient centrifugation, at high protein concentration, in presence of 0.05 mM 6-azauridine 5'-phosphate or 0.002 1-(5'-phospho-beta-D-ribofuranosyl)barbituric acid. The enzyme exists as monomer at low protein concentrations Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae 15C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, 20% glycerol, retention of full activity for more than 2 years Saccharomyces cerevisiae
4°C, stable for 16 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Orotidine 5'-phosphate
-
Saccharomyces cerevisiae UMP + CO2
-
?
Orotidine 5'-phosphate
-
Saccharomyces cerevisiae 15C UMP + CO2
-
?