Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(5'-phospho-beta-D-ribofuranosyl)barbituric acid | - |
Saccharomyces cerevisiae | |
6-azauridine 5'-phosphate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
orotidine 5'-phosphate | pH 6, 25°C | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29500 | - |
sucrose density gradient centrifugation, at low protein concentration, in absence of ligands that bind at the catalytic site. The enzyme exists as dimer at high protein concentrations | Saccharomyces cerevisiae |
64000 | - |
sucrose density gradient centrifugation, at high protein concentration, in presence of 0.05 mM 6-azauridine 5'-phosphate or 0.002 1-(5'-phospho-beta-D-ribofuranosyl)barbituric acid. The enzyme exists as monomer at low protein concentrations | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae 15C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, 20% glycerol, retention of full activity for more than 2 years | Saccharomyces cerevisiae |
4°C, stable for 16 months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | - |
Saccharomyces cerevisiae | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | - |
Saccharomyces cerevisiae 15C | UMP + CO2 | - |
? |