Cloned (Comment) | Organism |
---|---|
mutant enzymes expressed in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA517-656 | C-terminall truncated enzyme is fully active | Rattus norvegicus |
DELTA517-656/C104S | activity is significantly decreased relative to wild-type enzyme | Rattus norvegicus |
DELTA517-656/C115S | activity is significantly decreased relative to wild-type enzyme | Rattus norvegicus |
DELTA517-656/C254S | activity is significantly decreased relative to wild-type enzyme | Rattus norvegicus |
DELTA517-656/C316S | activity is significantly decreased relative to wild-type enzyme | Rattus norvegicus |
DELTA517-656/D276G | no activity detectable | Rattus norvegicus |
DELTA517-656/H197G | activity is 11.8fold lower than wild-type activity | Rattus norvegicus |
DELTA517-656/K308G | no activity detectable | Rattus norvegicus |
DELTA517-656/Q343G344ins | no activity detectable | Rattus norvegicus |
DELTA517-656/Q343ins | activity is 2.3fold lower than wild-type activity | Rattus norvegicus |
DELTA517-656/S307G | activity is 13.4fold lower than wild-type activity | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Rattus norvegicus |