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Literature summary for 4.1.1.2 extracted from

  • Zhu, W.; Reinhardt, L.A.; Richards, N.G.J.
    Second-shell hydrogen bond impacts transition-state structure in Bacillus subtilis oxalate decarboxylase (2018), Biochemistry, 57, 3425-3432 .
    View publication on PubMed
Search for more literature for 4.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
W132F the mutant with increased manganese content shows reduced activity compared to the wild type enzyme Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
 oxalate wild type enzyme, at pH 4.2 and 25°C Bacillus subtilis
27
-
 oxalate mutant enzyme W132F, at pH 4.2 and 25°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ dependent on Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxalate + H+ Bacillus subtilis
-
 formate + CO2
-
 ?
oxalate + H+ Bacillus subtilis 168
-
 formate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34714
-
-
Bacillus subtilis 168 O34714
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxalate + H+
-
 Bacillus subtilis formate + CO2
-
 ?
oxalate + H+
-
 Bacillus subtilis 168 formate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
OXDC
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60
-
 oxalate mutant enzyme W132F, at pH 4.2 and 25°C Bacillus subtilis
60
-
 oxalate wild type enzyme, at pH 4.2 and 25°C Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
 oxalate mutant enzyme W132F, at pH 4.2 and 25°C Bacillus subtilis
10
-
 oxalate wild type enzyme, at pH 4.2 and 25°C Bacillus subtilis