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Literature summary for 4.1.1.19 extracted from

  • Kera, K.; Nagayama, T.; Nanatani, K.; Saeki-Yamoto, C.; Tominaga, A.; Souma, S.; Miura, N.; Takeda, K.; Kayamori, S.; Ando, E.; Higashi, K.; Igarashi, K.; Uozumi, N.
    Reduction of spermidine content resulting from inactivation of two arginine decarboxylases increases biofilm formation in Synechocystis sp. strain PCC 6803 (2018), J. Bacteriol., 200, pii: e00664-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.19

Cloned(Commentary)

Cloned (Comment) Organism
gene ADC1, semi-quantitative RT-PCR enzyme expression analysis, Adc1 shows constitutive expression, recombinant expression of His-tagged isozyme in Escherichia coli Synechocystis sp. PCC 6803
gene ADC2, semi-quantitative RT-PCR enzyme expression analysis, Adc2 shows constitutive expression, recombinant expression of His-tagged isozyme in Escherichia coli Synechocystis sp. PCC 6803

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl Adc1 exhibits a 2.9fold and 2.2fold increase in activity in buffer containing 250 mM NaCl and 500 mM NaCl, respectively, compared to salt-free buffer. The NaCl-stimulation of the activities of Adc1 and Adc2 might account for the increase in agmatine concentration in Synechocystis under high salt conditions in vivo Synechocystis sp. PCC 6803
NaCl Adc2 exhibits a 14fold and 13fold increase in activity in buffer containing 250 mM NaCl and 500 mM NaCl, respectively, compared to salt-free buffer. The NaCl-stimulation of the activities of Adc1 and Adc2 might account for the increase in agmatine concentration in Synechocystis under high salt conditions in vivo Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine Synechocystis sp. PCC 6803
-
 agmatine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803 P72587
-
-
Synechocystis sp. PCC 6803 P74576
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme Adc1 from Escherichia coli by nickel affinity chromatography to homogeneity. Possible occurrence of N-terminal or C-terminal degradation of Adc1 during purification Synechocystis sp. PCC 6803
recombinant His-tagged isozyme Adc2 from Escherichia coli by nickel affinity chromatography to homogeneity. Possible occurrence of N-terminal or C-terminal degradation of Adc2 during purification Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine
-
 Synechocystis sp. PCC 6803 agmatine + CO2
-
 ?
additional information purified recombinant isozyme Adc2 shows high arginine decarboxylase activity compared to purified isozyme Adc1 Synechocystis sp. PCC 6803 ?
-
 ?
additional information purified recombinant isozyme Adc2 shows high arginine decarboxylase activity compared to purified isozyme Adc1. The absolute activity of Adc1, in absence of salt, is extremely low Synechocystis sp. PCC 6803 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 74500, about, sequence calcualtion of His-tagged isozyme Adc1 Synechocystis sp. PCC 6803
? x * 78200, about, sequence calcualtion of His-tagged isozyme Adc2 Synechocystis sp. PCC 6803

Synonyms

Synonyms Comment Organism
ADC1
-
 Synechocystis sp. PCC 6803
ADC2
-
 Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Synechocystis sp. PCC 6803
8
-
-
 Synechocystis sp. PCC 6803

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Synechocystis sp. PCC 6803

Expression

Organism Comment Expression
Synechocystis sp. PCC 6803 no effect of NaCl at 0.5 M on the expression of adc1 additional information
Synechocystis sp. PCC 6803 no effect of NaCl at 0.5 M on the expression of adc2 additional information

General Information

General Information Comment Organism
malfunction inactivation of two arginine decarboxylases results in reduction of spermidine content and increases biofilm formation in Synechocystis sp. strain PCC 6803. Disruption of the adc genes in Synechocystis results in formation of biofilms even under non-stress conditions, e.g. the absence of salt, while salt stress decreases polyamine content in Synechocystis Synechocystis sp. PCC 6803
malfunction inactivation of two arginine decarboxylases results in reduction of spermidine content and increases biofilm formation in Synechocystis sp. strain PCC 6803. Disruption of the adc genes in Synechocystis results in formation of biofilms even under non-stress conditions, e.g. the absence of salt, while salt stress decreases the polyamine content in Synechocystis Synechocystis sp. PCC 6803
metabolism bacteria and plants possess an alternative pathway for putrescine biosynthesis from arginine through the action of arginine decarboxylase (Adc) catalyzing the initial step. The product of that reaction, agmatine, is subsequently converted to putrescine, spermidine, and spermine via a series of steps in the polyamine synthetic pathway. The two arginine decarboxylases, isozymes Adc1 and Adc2, possess function in the polyamine synthesis pathway, there is a negative correlation between biofilm formation and polyamine content Synechocystis sp. PCC 6803
physiological function of the two isozymes in Synechocystis sp. PCC 6803, Adc2 has high arginine decarboxylase activity, whereas Adc1 is much less active Synechocystis sp. PCC 6803
physiological function of the two isozymes in Synechocystis sp. PCC 6803, Adc2 has high arginine decarboxylase activity, whereas Adc1 is much less active. Adc2 is the major arginine decarboxylase, Adc2 activity leads to inhibition of biofilm formation Synechocystis sp. PCC 6803