Cloned (Comment) | Organism |
---|---|
gene adiA, expression of untagged and N-terminally His10-tagged wild-type enzyme and inactivated mutant in strain BW25113 | Escherichia coli |
gene CPn1032, expression of untagged and N-terminally His10-tagged enzyme in Escherichia coli strain Bl21(DE3), CPn1032 expression complements the adiA null mutation in Escherichia coli strain DEG0121, which shows low arginine decarboxylase enzyme activity and is deficient in AdiC transporter | Chlamydia pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an enzyme deletion null mutant, complementation by expression of the enzyme from Chlamydophila pneumoniae | Escherichia coli |
T52S | site-directed mutagenesis, the mutant enzyme is significantly impaired in proteolytic self-cleavage | Chlamydia pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-argininamide | 33% inhibition at 2 mM | Chlamydia pneumoniae | |
O-methyl hydroxylamine | 69% inhibition at 2 mM | Chlamydia pneumoniae | |
O-nitrobenzylhydroxylamine | 75% inhibition at 2 mM | Chlamydia pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Chlamydia pneumoniae | |
0.03 | - |
L-arginine | pH 8.4, 37°C, constitutive ArgDC | Escherichia coli | |
0.65 | - |
L-arginine | pH 5.2, 37°C, inducible ArgDC | Escherichia coli | |
5 | - |
L-arginine | pH 3.5, 37°C | Chlamydia pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
9000 | - |
3 * 16000, alpha subunit, + 3 * 9000, beta-subunit, trimeric dimer, (alpha/beta)3, SDS-PAGE | Chlamydia pneumoniae |
16000 | - |
3 * 16000, alpha subunit, + 3 * 9000, beta-subunit, trimeric dimer, (alpha/beta)3, SDS-PAGE | Chlamydia pneumoniae |
88000 | - |
recombinant enzyme, gel filtration | Chlamydia pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | Chlamydia pneumoniae | - |
agmatine + CO2 | - |
? | |
L-arginine | Escherichia coli | the AdiA enzyme is required by the AR3 arginine-dependent acid resistance system | agmatine + CO2 | - |
? | |
L-arginine | Chlamydia pneumoniae Kajaani 6 | - |
agmatine + CO2 | - |
? | |
additional information | Chlamydia pneumoniae | acting together with a putative arginine-agmatine antiporter, the CPn1032 homologs may have evolved convergently to form an arginine-dependent acid resistance system, this system could reduce the host cell arginine concentration and produce inhibitors of nitric oxide synthase, obligately intracellular chlamydiae may encounter acidic conditions, overview | ? | - |
? | |
additional information | Chlamydia pneumoniae Kajaani 6 | acting together with a putative arginine-agmatine antiporter, the CPn1032 homologs may have evolved convergently to form an arginine-dependent acid resistance system, this system could reduce the host cell arginine concentration and produce inhibitors of nitric oxide synthase, obligately intracellular chlamydiae may encounter acidic conditions, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydia pneumoniae | - |
gene CPn1032 | - |
Chlamydia pneumoniae Kajaani 6 | - |
gene CPn1032 | - |
Escherichia coli | P28629 | gene adiA, inducible and constitutive ArgDCs | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the recombinant enzyme self-cleaved to form a reactive pyruvoyl group, and the subunits assembled into a thermostable (alpha/beta)3 complex | Chlamydia pneumoniae |
Purification (Comment) | Organism |
---|---|
recombinant His10-tagged enzyme from Escherichia coli strain Bl21(DE3) by nickel affinity chromatography, recombinant untagged enzyme from Escherichia coli strain Bl21(DE3) by anion exchange chromatography, most of the protein is found in the insoluble portion of cell lysate | Chlamydia pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
arginine-dependent acid resistance assay, overview | Chlamydia pneumoniae |
8.1 | - |
purified recombinant enzyme | Chlamydia pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | - |
Chlamydia pneumoniae | agmatine + CO2 | - |
? | |
L-arginine | - |
Escherichia coli | agmatine + CO2 | - |
? | |
L-arginine | the AdiA enzyme is required by the AR3 arginine-dependent acid resistance system | Escherichia coli | agmatine + CO2 | - |
? | |
L-arginine | - |
Chlamydia pneumoniae Kajaani 6 | agmatine + CO2 | - |
? | |
L-canavanine | - |
Chlamydia pneumoniae | gamma-guanidinoxypropylamine + CO2 | - |
? | |
L-canavanine | - |
Chlamydia pneumoniae Kajaani 6 | gamma-guanidinoxypropylamine + CO2 | - |
? | |
additional information | acting together with a putative arginine-agmatine antiporter, the CPn1032 homologs may have evolved convergently to form an arginine-dependent acid resistance system, this system could reduce the host cell arginine concentration and produce inhibitors of nitric oxide synthase, obligately intracellular chlamydiae may encounter acidic conditions, overview | Chlamydia pneumoniae | ? | - |
? | |
additional information | the enzyme is highly substrate specific, no activity with D-arginine, L-aspartate, L-citrulline, L-glutamine, L-histidine, L-homoarginine, L-lysine, N-methyl-Larginine, and L-ornithine | Chlamydia pneumoniae | ? | - |
? | |
additional information | acting together with a putative arginine-agmatine antiporter, the CPn1032 homologs may have evolved convergently to form an arginine-dependent acid resistance system, this system could reduce the host cell arginine concentration and produce inhibitors of nitric oxide synthase, obligately intracellular chlamydiae may encounter acidic conditions, overview | Chlamydia pneumoniae Kajaani 6 | ? | - |
? | |
additional information | the enzyme is highly substrate specific, no activity with D-arginine, L-aspartate, L-citrulline, L-glutamine, L-histidine, L-homoarginine, L-lysine, N-methyl-Larginine, and L-ornithine | Chlamydia pneumoniae Kajaani 6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 3 * 16000, alpha subunit, + 3 * 9000, beta-subunit, trimeric dimer, (alpha/beta)3, SDS-PAGE | Chlamydia pneumoniae |
More | the recombinant enzyme self-cleaved to form a reactive pyruvoyl group, and the subunits assembled into a thermostable (alpha/beta)3 complex | Chlamydia pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
AdiA | - |
Escherichia coli |
ARGDC | - |
Chlamydia pneumoniae |
ARGDC | - |
Escherichia coli |
CPn1032 homolog | - |
Chlamydia pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
47 | - |
- |
Chlamydia pneumoniae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | about 50% of maximal activity at 20°C and 80°C | Chlamydia pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, the purified enzyme retains 48% of maximal activity | Chlamydia pneumoniae |
100 | - |
10 min, the purified enzyme retains 13% of maximal activity | Chlamydia pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.9 | - |
L-arginine | pH 3.5, 37°C | Chlamydia pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.4 | - |
- |
Chlamydia pneumoniae |
5.2 | - |
inducible ArgDC | Escherichia coli |
8.4 | - |
constitutive ArgDC | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1.5 | 5.5 | activity range | Chlamydia pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | The serine residue at N terminus of the {alpha} subunit forms a reactive pyruvoyl group that functions analogously to pyridoxal 5'-phosphate | Chlamydia pneumoniae | |
pyridoxal 5'-phosphate | dependent on | Escherichia coli |