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Literature summary for 4.1.1.18 extracted from
- Alkaliphilic lysine decarboxylases for effective synthesis of cadaverine from L-lysine (2016), Korean J. Chem. Engin., 33, 1530-1533 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme can potentially be used for synthesis and production of cadaverine. Cadaverine is a promising chemical platform that has a variety of applications, including the production of polyamides, polyurethanes, chelating agents, and additives. In particular, it is relevant for the production of bio-based nylon, which can be used to replace conventional polyamides from petrochemical routes. Whereas Nylon 66 is a polymer composed of hexamethylenediamine and adipic acid, Nylon 56 is a polymer that is produced by the co-polymerization of cadaverine and adipic acid | Geobacillus thermodenitrificans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gtLDC, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Geobacillus thermodenitrificans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine | Geobacillus thermodenitrificans | - |
cadaverine + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermodenitrificans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Geobacillus thermodenitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine | - |
Geobacillus thermodenitrificans | cadaverine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54800, recombinant His6-tagged enzyme, SDS-PAGE | Geobacillus thermodenitrificans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gtLDC | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Geobacillus thermodenitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
recombinant enzyme | Geobacillus thermodenitrificans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | enzyme gtLDC does not exhibit effective activity in alkaline pH conditions, the conversion rate of about 8% is similar at pH 8.0 and pH 9.0, no activity at pH 10.0 | Geobacillus thermodenitrificans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Geobacillus thermodenitrificans | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | compared to the activity of lysine/ornithine decarboxylase from Selenomonas ruminantium and from Vibrio vulnificus, the cadaverine producing activity of enzyme gtLDC is severalfold reduced | Geobacillus thermodenitrificans |
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Release 2023.1 (January 2023)
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