Literature summary for 4.1.1.17 extracted from

Yadav, A.; Amit, A.; Chaudhary, R.; Chandel, A.S.; Mahantesh, V.; Suman, S.S.; Singh, S.K.; Dikhit, M.R.; Ali, V.; Rabidas, V.; Pandey, K.; Kumar, A.; Das, P.; Bimal, S.
Leishmania donovani impairment of the cellular immune response against recombinant ornithine decarboxylase protein as a possible evasion strategy of Leishmania in visceral leishmaniasis (2015), Int. J. Parasitol., 45, 33-42 .

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Application

Application	Comment	Organism
drug development	because ODC is essential for trypanothione biosynthesis, polyamines of parasitic protozoa may be potential targets for the development of drugs with activity against Leishmania donovani. One goal of drug development is to evaluate the potential of Leishmania antigen for evoking a Th1 response against Leishmania infection	Leishmania donovani

Cloned(Commentary)

Cloned (Comment)	Organism
gene odc, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21	Leishmania donovani

Inhibitors

Inhibitors	Comment	Organism	Structure
L-alpha-difluoromethylornithine	-	Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-ornithine	Leishmania donovani	-	putrescine + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Leishmania donovani	P27116	prepared from a Leishmania donovani clinical strain procured from a patient admitted to the Rajendra Medical Research Institute of Medical Science, Patna, India as described by Gupta et al. (2007)	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis	Leishmania donovani

Source Tissue

Source Tissue	Comment	Organism	Textmining
amastigote	-	Leishmania donovani	-
additional information	significantly higher growth of Leishmania donovani parasites in the presence of recombinant LdODC compared with the parasite culture in the absence of any stimulation of both promastigotes and amastigotes, although it is higher in amastigotes	Leishmania donovani	-
promastigote	-	Leishmania donovani	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-ornithine	-	Leishmania donovani	putrescine + CO2	-	?

Subunits

Subunits	Comment	Organism
?	x * 77000, about, recombinant His-tagged enzyme, SDS-PAGE	Leishmania donovani

Synonyms

Synonyms	Comment	Organism
LdODC	-	Leishmania donovani
ODC	-	Leishmania donovani

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Leishmania donovani

General Information

General Information	Comment	Organism
malfunction	use of enzyme inhibitor alpha-difluoromethylornithine can reduce the activity of ornithine decarboxylase of Leishmannia donovani eliminating the parasite-induced immune suppression and inducing collateral host protective responses in visceral leishmaniasis. Inhibition of r-LdODC can direct the active IFN-c dominant immune responses necessary for parasite clearance in VL patients	Leishmania donovani
physiological function	ODC is essential for trypanothione biosynthesis. Trypanothione is the major reduced thiol which is responsible for the modulation of the immune response and pathogenesis in visceral leishmaniasis (VL). Analysis of the host protective role of an anti-ODC CD4+ T cell response and anti-leishmanial MU function in VL patients, relationship between ornithine decarboxylase and the cellular immune response in VL patients, overview. Pivotal role for recombinant LdODC in causing strong immune suppression in a susceptible host. Recombinant LdODC-induced CD4+ and CD8+ T-cell responses, functional profiles and effects on IFN-gamma and interleukin-10 levels, overview	Leishmania donovani

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Release 2023.1 (January 2023)