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Literature summary for 4.1.1.17 extracted from
- Identification of essential active-site residues in ornithine decarboxylase of Nicotiana glutinosa decarboxylating both L-ornithine and L-lysine (2001), Biochem. J., 360, 657-665.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Nicotiana glutinosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C338A | 14% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
| C377A | 4% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
| C96A | 42% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
| K95A | 1% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | 0.001 mM, approx. 80% inhibition of ODC activity after 8 min | Nicotiana glutinosa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.56 | - |
L-ornithine | - |
Nicotiana glutinosa | |
| 1.59 | - |
L-lysine | - |
Nicotiana glutinosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46500 | - |
2 * 46500, SDS-PAGE | Nicotiana glutinosa |
| 92000 | - |
native PAGE | Nicotiana glutinosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine | Nicotiana glutinosa | - |
1,5-diaminopentane + CO2 | - |
? | |
| L-ornithine | Nicotiana glutinosa | - |
putrescine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana glutinosa | Q9FPK5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant ODC | Nicotiana glutinosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine | - |
Nicotiana glutinosa | 1,5-diaminopentane + CO2 | - |
? | |
| L-ornithine | - |
Nicotiana glutinosa | putrescine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46500, SDS-PAGE | Nicotiana glutinosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ODC | - |
Nicotiana glutinosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
half-life 70 min | Nicotiana glutinosa |
| 50 | - |
half-life 20 min | Nicotiana glutinosa |
| 60 | - |
half-life 5 min | Nicotiana glutinosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
L-lysine | - |
Nicotiana glutinosa | |
| 77.8 | - |
L-ornithine | - |
Nicotiana glutinosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
L-lysine decarboxylation | Nicotiana glutinosa |
| 8 | - |
L-ornithine decarboxylation | Nicotiana glutinosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | L-lysine decarboxylation, approx. 40% of maximal activity at pH 7.5 | Nicotiana glutinosa |
| 7.2 | 8.7 | L-ornithine decarboxylation, approx. 35% of maximal activity at pH 7.2 | Nicotiana glutinosa |
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Release 2023.1 (January 2023)
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