Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dGTP | reactivation of ODC at pH values above 7.0 | Lactobacillus sp. 30a | |
GDP | reactivation of ODC at pH values above 7.0 | Lactobacillus sp. 30a | |
GTP | reactivation of ODC at pH values above 7.0, dodecameric ODC binds 2 GTPs per dimer at pH 5.8 | Lactobacillus sp. 30a |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lactobacillus sp. 30a |
Crystallization (Comment) | Organism |
---|---|
G121Y mutant dimer at pH 7.0 in the presence of GTP, purified ODC is dialyzed against 100 mM sodium-HCl, pH 6.5, 1 mM dithiothreitol, 0.2 mM pyridoxal 5'-phosphate, 0.02% sodium azide and 0.5 mM EDTA and concentrated to 20 mg/ml, crystals are grown at room temperature by the sitting-drop method from reservoirs containing 30% polyethylene glycol 3350, 200 mM ammonium acetate and 100 mM Na-HEPES, pH 7.0, crystals diffract to 2.7 A resolution | Lactobacillus sp. 30a |
Protein Variants | Comment | Organism |
---|---|---|
G121Y | Gly121 is buried in the dimer-dimer interface, mutation of Gly121 into Tyr prevents association of dimers into dodecamers, G121Y shows similar activity as native ODC | Lactobacillus sp. 30a |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
12 * 83000, SDS-PAGE, dodecamer composed of six homodimers | Lactobacillus sp. 30a |
990600 | - |
- |
Lactobacillus sp. 30a |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | Lactobacillus sp. 30a | - |
putrescine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus sp. 30a | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant ODC | Lactobacillus sp. 30a |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | - |
Lactobacillus sp. 30a | putrescine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 83000, SDS-PAGE, dodecamer composed of six homodimers | Lactobacillus sp. 30a |
Synonyms | Comment | Organism |
---|---|---|
ODC | - |
Lactobacillus sp. 30a |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
- |
Lactobacillus sp. 30a |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Lactobacillus sp. 30a |