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Literature summary for 4.1.1.15 extracted from

  • Kanwal, S.; Rastogi, R.; Incharoensakdi, A.
    Glutamate decarboxylase activity and gamma-aminobutyric acid content in Synechocystis sp. PCC 6803 under osmotic stress and different carbon sources (2014), J. Appl. Phycol., 26, 2327–2333.
No PubMed abstract available
Search for more literature for 4.1.1.15

Cloned(Commentary)

Cloned (Comment) Organism
gene gad, RT-PCR expression analysis Synechocystis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Synechocystis sp. 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Synechocystis sp.
-
 4-aminobutanoate + CO2
-
 ir

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 gene gad
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
 Synechocystis sp. 4-aminobutanoate + CO2
-
 ir

Synonyms

Synonyms Comment Organism
GAD
-
 Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 assay at Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Synechocystis sp.

Expression

Organism Comment Expression
Synechocystis sp. 4-aminobutanoate content is enhanced by osmotic stress induced by both sorbitol and NaCl up to 100 and 50 mM, respectively. D-Glucose is also corroborated by the increase in the levels of gad transcripts. GAD activity increases by fourfold when late log phase cells are grown mixotrophically for 24 h in medium containing 0.1% glucose as carbon source, whereas a nearly fivefold increase in 4-aminobutanoate content is observed under the same condition up

General Information

General Information Comment Organism
metabolism the 4-aminobutanoate shunt pathway possesses three enzymes encoded by genes gad, gabT, and gabD. Among the three, GAD is the key cytosolic-located enzyme which catalyzes the irreversible decarboxylation of L-glutamate to produce 4-aminobutanoate Synechocystis sp.