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Literature summary for 4.1.1.15 extracted from
- Purification, properties and cDNA cloning of glutamate decarboxylase in germinated faba bean (Vicia faba L.) (2013), Food Chem., 138, 1945-1951.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gad, cloning from germinated faba bean, DNA and amino acid sequence determination and analysis, sequence comparisons | Vicia faba |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Vicia faba |  |
| Aminoxyacetate | - |
Vicia faba | |
| Ba2+ | - |
Vicia faba | |
| Ca2+ | activates at 0.2 mM, slight inhibition above 1 mM | Vicia faba | |
| Cu2+ | strong inhibition at 3 mM | Vicia faba | |
| EGTA | - |
Vicia faba | |
| Fe3+ | - |
Vicia faba | |
| L-cysteine | - |
Vicia faba | |
| Mg2+ | - |
Vicia faba | |
| Na2EDTA | - |
Vicia faba | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.63 | - |
L-glutamate | pH 6.0, 40°C, recombinant enzyme | Vicia faba | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates at 0.2 mM, slight inhibition above 1 mM | Vicia faba | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57740 | - |
x * 57740, sequence calculation, x * 58000, SDS-PAGE | Vicia faba |
| 58000 | - |
x * 57740, sequence calculation, x * 58000, SDS-PAGE | Vicia faba |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | Vicia faba | - |
4-aminobutanoate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vicia faba | M1GCP8 | cv. Qidou 2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 32fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography | Vicia faba |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.44 | - |
purified native enzyme, pH 6.0, 40°C | Vicia faba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | - |
Vicia faba | 4-aminobutanoate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57740, sequence calculation, x * 58000, SDS-PAGE | Vicia faba |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAD | - |
Vicia faba |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant enzyme | Vicia faba |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | activity range, profile overview, inactivation above | Vicia faba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
recombinant enzyme | Vicia faba |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | activity range, profile overview | Vicia faba |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Vicia faba | sequence calculation | - |
5.41 |
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