Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | at a separate concentration of Ca2+ and calmodulin of 0.2 mM and 150 nM, the rice bran GAD is significantly enhanced 3fold. Addition of exogenous EGTA or TFP efficiently inhibited the stimulatory effect of Ca2+/CaM complex | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at a separate concentration of Ca2+ and calmodulin of 0.2 mM and 150 nM, the rice bran GAD is significantly enhanced 3fold | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration | Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.009 | - |
crude extract, in 50 mM PBS buffer, pH 5.6, at 40°C | Oryza sativa |
7.666 | - |
after 851.7fold purification, in 50 mM PBS buffer, pH 5.6, at 40°C | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Oryza sativa | 4-aminobutanoate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAD | - |
Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Oryza sativa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
the GAD activity determined at pH 7.0 in the absence of Ca2+/calmodulin is about 70% of the maximal activity at 5.6 | Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Oryza sativa |