Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 58.3% residual activity at 2 mM | Oryza sativa | |
Al3+ | 91.2% residual activity at 2 mM | Oryza sativa | |
CH3COOH | 57.4% residual activity at 2 mM | Oryza sativa | |
EDTA | 91.2% residual activity at 2 mM | Oryza sativa | |
Hg2+ | 37.7% residual activity at 2 mM | Oryza sativa | |
Mg2+ | 90.1% residual activity at 2 mM | Oryza sativa | |
Mn2+ | 92.6% residual activity at 2 mM | Oryza sativa | |
SDS | 66.9% residual activity at 40 mM | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0274 | - |
L-glutamate | - |
Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | GAD activity is significantly enhanced in the presence of Ca2+ | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
SDS-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q9AR41 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, DEAE Sepharose column chromatography, and Superdex 200 gel filtration | Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.68 | - |
- |
Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Oryza sativa | 4-aminobutanoate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAD | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Oryza sativa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | retains 78 and 69% of its maximum activity at 30 and 50°C, respectively | Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | the enzyme is significantly inactivated when the temperature is increased to 60°C, with 36 and 49% of the original activity being lost after 30 and 60 min of incubation respectively, and is completely inactivated at 70°C | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Oryza sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | retains 80% of its original activity in the pH range 5-9 | Oryza sativa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
beyond pH range 5.0-9.0 a sharp decline in enzyme stability is observed, and negligible activity is detected at pH above 10 | Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Oryza sativa |