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Literature summary for 4.1.1.15 extracted from

  • Wang, L.; Xu, D.X.; Lv, Y.G.; Zhang, H.
    Purification and biochemical characterisation of a novel glutamate decarboxylase from rice bran (2010), J. Sci. Food Agric., 90, 1027-1033.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 58.3% residual activity at 2 mM Oryza sativa
Al3+ 91.2% residual activity at 2 mM Oryza sativa
CH3COOH 57.4% residual activity at 2 mM Oryza sativa
EDTA 91.2% residual activity at 2 mM Oryza sativa
Hg2+ 37.7% residual activity at 2 mM Oryza sativa
Mg2+ 90.1% residual activity at 2 mM Oryza sativa
Mn2+ 92.6% residual activity at 2 mM Oryza sativa
SDS 66.9% residual activity at 40 mM Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0274
-
L-glutamate
-
Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ GAD activity is significantly enhanced in the presence of Ca2+ Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
SDS-PAGE Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q9AR41
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, DEAE Sepharose column chromatography, and Superdex 200 gel filtration Oryza sativa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.68
-
-
Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
Oryza sativa 4-aminobutanoate + CO2
-
?

Synonyms

Synonyms Comment Organism
GAD
-
Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Oryza sativa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 retains 78 and 69% of its maximum activity at 30 and 50°C, respectively Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 the enzyme is significantly inactivated when the temperature is increased to 60°C, with 36 and 49% of the original activity being lost after 30 and 60 min of incubation respectively, and is completely inactivated at 70°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
5 9 retains 80% of its original activity in the pH range 5-9 Oryza sativa

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
beyond pH range 5.0-9.0 a sharp decline in enzyme stability is observed, and negligible activity is detected at pH above 10 Oryza sativa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Oryza sativa