Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.15 extracted from

  • Awad, R.; Crump, K.; Mullally, M.; Sardana, R.K.; Arnason, J.T.; Trudeau, V.L.
    An improved method for production of recombinant human glutamic acid decarboxylase 65 for use in phytopharmaceutical assessment (2008), Pharm. Biol., 46, 72-81.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
asiaticoside 20% activation at 0.01 mg/ml Homo sapiens
asiaticoside an increase in GAD65 activity by 22% is observed with asiaticoside Homo sapiens
asiaticoside an increase in GAD65 activity by 4.5% is observed with asiaticoside Homo sapiens
bilobalide 20% activation at 0.01 mg/ml Homo sapiens
bilobalide an increase in GAD65 activity by approximately 18% is observed with bilobalide Homo sapiens
bilobalide an increase in GAD65 activity by 17.8% is observed with bilobalide Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens
expressed in Escherichia coli DH5alpha cells Homo sapiens
gene gadB, expression in Escherichia coli strain DH5alpha using plasmids pMAL-c2X and the pGEX-3X and fusion to the maltose binding protein or GST, cytoplasmic targeting, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(E)-pent-2-enoic acid 20% inhibition at 0.01 mg/ml Homo sapiens
3-Mercaptopropionic acid 43% inhibition at 0.1 mM Homo sapiens
allylglycine
-
Homo sapiens
asiaticoside
-
Homo sapiens
betulinic acid 27% inhibition at 0.01 mg/ml Homo sapiens
bilobalide
-
Homo sapiens
ginsenosides 23% inhibition at 0.01 mg/ml, ethanol extract of Panax quinquefolius, different glycoside derivatives, overview Homo sapiens
additional information aasiaticoside does not inhibit GAD activity even at the highest dose of 0.03 mg/ml. Allylglycine does not inhibit GAD activity even at the highest dose of 0.1 mM; asiaticoside does not inhibit GAD activity even at the highest dose of 0.03 mg/ml, allylglycine exhibits no enzyme inhibition even at the highest dose of 0.1 mM; minor inhibition is seen with the ethanol extract of Panax quinquefolius (23%), betulinic acid (27%), and valerenic acid (20%); minor inhibition is seen with the ethanol extract of Panax quinquefolius (ginsenosides) (23%) Homo sapiens
ursolic acid 11.2% inhibition at 0.01 mg/ml Homo sapiens
valerenic acid 20% inhibition at 0.01 mg/ml Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Homo sapiens
-
4-aminobutanoate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography, glutathione Sepharose 4B resin column chromatography Homo sapiens
glutathione sepharose 4B resin column chromatography and amylose resin column chromatography Homo sapiens
recombinant tagged enzyme from Escherichia coli strain DH5alpha Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
nervous system two isoforms ofGAD, GAD67and GAD65 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
Homo sapiens 4-aminobutanoate + CO2
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
GAD65
-
Homo sapiens
GAD65 isoform Homo sapiens
glutamic acid decarboxylase 65
-
Homo sapiens
glutamic acid decarboxylase 65 isoform Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Homo sapiens
pyridoxal 5'-phosphate dependent on Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0123
-
-
Homo sapiens 3-Mercaptopropionic acid
0.0123
-
at 37°C Homo sapiens 3-Mercaptopropionic acid
0.0123
-
at 37°C, pH not specified in the publication Homo sapiens 3-Mercaptopropionic acid