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Literature summary for 4.1.1.15 extracted from
- Purification and characterization of the first archaeal glutamate decarboxylase from Pyrococcus horikoshii (2009), Biosci. Biotechnol. Biochem., 73, 224-227.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression and sequence comparisons | Pyrococcus horikoshii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | development of a hyperthermostable enzyme for industrial applications, overview | Pyrococcus horikoshii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
1 * 42000, SDS-PAGE | Pyrococcus horikoshii |
| 43000 | - |
gel filtration | Pyrococcus horikoshii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | Pyrococcus horikoshii | - |
4-aminobutanoate + CO2 | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | - |
gene PH0937 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme by ammonium sulfate fractionation, hydrophobic interaction chromatography, and dialysis | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | - |
Pyrococcus horikoshii | 4-aminobutanoate + CO2 | - |
ir | |
| additional information | the recombinant engineered enzyme shows a broad substrate specificity | Pyrococcus horikoshii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Pyrococcus horikoshii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAD | - |
Pyrococcus horikoshii |
| More | GAD belongs to a subset of pyridoxal 5'-phosphate-dependent enzymes classified as group 2 | Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 97 | - |
above, recombinant engineered enzyme | Pyrococcus horikoshii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
purified recombinant enzyme, stable | Pyrococcus horikoshii |
| 95 | - |
purified recombinant enzyme, half-life is 3 h | Pyrococcus horikoshii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant engineered enzyme | Pyrococcus horikoshii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Pyrococcus horikoshii | |
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