Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 24fold activation of wild-type in presence of both Ca2+ and calmodulin. For mutant lacking 19 amino acids at N-terminus, 12.9fold activation. Mutant lacking 9 amino acids at C-terminus, complete loss of activation | Petunia sp. | |
Calmodulin | 24fold activation of wild-type in presence of both Ca2+ and calmodulin. For mutant lacking 19 amino acids at N-terminus, 12.9fold activation. Mutant lacking 9 amino acids at C-terminus, complete loss of activation | Petunia sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Petunia sp. |
Protein Variants | Comment | Organism |
---|---|---|
DELTAC9 | 9 amino acid C-terminal deletion mutant, no formation of complexes larger than 340 kDa, no activation by Ca2+/calmodulin. The ability to bind calmodulin in the presence of Ca2+ is retained, and mutant may be purified by calmodulin affinity chroamtography. 12% of wild-type activity at pH 5.8 | Petunia sp. |
DELTAN18 | 18 amino acid N-terminal deletion mutant, activation by Ca2+/calmodulin is reduced by about 50%. 40% of wild-type activity at pH 5.8 | Petunia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, SDS-PAGE of full-length enzyme | Petunia sp. |
300000 | - |
gel filtration, absence of Ca2+/calmodulin | Petunia sp. |
580000 | - |
gel filtration, absence of Ca2+/calmodulin | Petunia sp. |
680000 | - |
gel filtration, presence of Ca2+/calmodulin | Petunia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia sp. | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 58000, SDS-PAGE of full-length enzyme | Petunia sp. |