BRENDA - Enzyme Database
show all sequences of 4.1.1.112

Biochemical and genetic characterization of the Enterococcus faecalis oxaloacetate decarboxylase complex

Repizo, G.D.; Blancato, V.S.; Mortera, P.; Lolkema, J.S.; Magni, C.; Appl. Environ. Microbiol. 79, 2882-2890 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of gene oadH as maltose-binding protein fusion protein in Escherichia coli, genes oadA-oadD, expression of recombinant His-tagged subunits in Escherichia coli strain BL21(DE3)
Enterococcus faecalis
Engineering
Amino acid exchange
Commentary
Organism
additional information
oadA, oadB, and oadD deletion mutants are constructed, while an oadH mutant strain cannot be obtained in spite of using different genetic strategies
Enterococcus faecalis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane
Enterococcus faecalis
5737
-
membrane
Ef-B is a membrane-bound subunit, while subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane
Enterococcus faecalis
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Oxaloacetate
Enterococcus faecalis
-
Pyruvate + CO2
-
-
?
Oxaloacetate
Enterococcus faecalis JH2-2
-
Pyruvate + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Enterococcus faecalis
-
genes oadA, oadB, oadC, and oadD
-
Enterococcus faecalis JH2-2
-
genes oadA, oadB, oadC, and oadD
-
Purification (Commentary)
Commentary
Organism
recombinnat maltose-binding protein fusion protein OadH from Escherichia coli by amylase affinity chromatography, recombinant His-tagged subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Enterococcus faecalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Oxaloacetate
-
726737
Enterococcus faecalis
Pyruvate + CO2
-
-
-
?
Oxaloacetate
-
726737
Enterococcus faecalis JH2-2
Pyruvate + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
the enzyme is constituted of four subunits: catalytic subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and subunit Ef-H. Subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane. The Ef-H subunit is involved in the cytoplasmic Ef-AHD complex formation. Analysis of subunit interactions and complex formation, overview
Enterococcus faecalis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of gene oadH as maltose-binding protein fusion protein in Escherichia coli, genes oadA-oadD, expression of recombinant His-tagged subunits in Escherichia coli strain BL21(DE3)
Enterococcus faecalis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
oadA, oadB, and oadD deletion mutants are constructed, while an oadH mutant strain cannot be obtained in spite of using different genetic strategies
Enterococcus faecalis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane
Enterococcus faecalis
5737
-
membrane
Ef-B is a membrane-bound subunit, while subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane
Enterococcus faecalis
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Oxaloacetate
Enterococcus faecalis
-
Pyruvate + CO2
-
-
?
Oxaloacetate
Enterococcus faecalis JH2-2
-
Pyruvate + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinnat maltose-binding protein fusion protein OadH from Escherichia coli by amylase affinity chromatography, recombinant His-tagged subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Enterococcus faecalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Oxaloacetate
-
726737
Enterococcus faecalis
Pyruvate + CO2
-
-
-
?
Oxaloacetate
-
726737
Enterococcus faecalis JH2-2
Pyruvate + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
the enzyme is constituted of four subunits: catalytic subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and subunit Ef-H. Subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane. The Ef-H subunit is involved in the cytoplasmic Ef-AHD complex formation. Analysis of subunit interactions and complex formation, overview
Enterococcus faecalis
General Information
General Information
Commentary
Organism
physiological function
presence of the membrane-bound Ef-B subunit is required for full alkalinization of the internal medium of Enterococcus faecalis cells during citrate fermentation
Enterococcus faecalis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
presence of the membrane-bound Ef-B subunit is required for full alkalinization of the internal medium of Enterococcus faecalis cells during citrate fermentation
Enterococcus faecalis
Other publictions for EC 4.1.1.112
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748206
Pircher
Identification of FAH domain- ...
Homo sapiens
J. Biol. Chem.
290
6755-6762
2015
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-
1
-
3
-
1
1
-
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2
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-
-
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726821
Lietzan
Functionally diverse biotin-de ...
Klebsiella aerogenes, Vibrio cholerae
Arch. Biochem. Biophys.
544
75-86
2014
-
-
-
-
-
-
-
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2
-
4
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5
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5
5
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726737
Repizo
Biochemical and genetic charac ...
Enterococcus faecalis, Enterococcus faecalis JH2-2
Appl. Environ. Microbiol.
79
2882-2890
2013
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1
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713674
Ran
Expression, purification, crys ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
67
968-970
2011
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-
1
1
-
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2
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1
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-
-
-
-
-
-
-
-
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714979
Espariz
Identification of malic and so ...
Enterococcus faecalis
FEBS J.
278
2140-2151
2011
-
-
1
-
-
-
10
1
-
1
-
-
-
1
-
-
1
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1
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1
1
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10
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1
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1
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1
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1
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1
1
1
-
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-
2
2
-
1
1
715662
Balsera
Quaternary structure of the ox ...
Vibrio cholerae
J. Biol. Chem.
286
9457-9467
2011
-
-
1
-
-
-
-
-
1
1
1
-
-
2
-
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704327
Klaffl
Genetic and functional analysi ...
Corynebacterium glutamicum
J. Bacteriol.
192
2604-2612
2010
-
-
-
-
-
-
1
1
1
-
3
-
-
2
-
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1
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1
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1
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1
1
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716673
Granjon
Structure-function relations i ...
Vibrio cholerae
PLoS ONE
5
e10935
2010
-
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1
-
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-
2
-
1
1
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3
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1
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-
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1
1
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-
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678331
Narayanan
Structure and function of PA48 ...
Pseudomonas aeruginosa
Biochemistry
47
167-182
2008
-
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1
1
3
-
6
5
-
1
-
-
-
3
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1
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6
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1
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2
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692317
Dharmarajan
Tyr235 of human cytosolic phos ...
Homo sapiens
FEBS J.
275
5810-5819
2008
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1
-
3
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2
1
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1
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1
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1
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3
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1
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1
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1
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692806
Augagneur
Mutation of the oxaloacetate d ...
Lactococcus lactis
J. Appl. Microbiol.
104
260-268
2008
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1
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692884
Blancato
Transcriptional regulation of ...
Enterococcus faecalis
J. Bacteriol.
190
7419-7430
2008
1
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681424
Studer
Crystal structure of the carbo ...
Vibrio cholerae
J. Mol. Biol.
367
547-557
2007
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1
1
10
-
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1
1
3
-
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2
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10
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679954
Kim
-
Determining citrate in fruit j ...
Pseudomonas sp.
Food Chem.
99
851-857
2006
-
-
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-
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-
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-
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1
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663796
Dahinden
Oxaloacetate decarboxylase of ...
Vibrio cholerae, Vibrio cholerae O395-N1
Arch. Microbiol.
183
121-129
2005
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1
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2
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1
1
2
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16
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1
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1
1
1
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1
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1
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664904
Dahinden
Identification of a domain in ...
Vibrio cholerae
FEBS J.
272
846-855
2005
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1
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663795
Dahinden
Oxaloacetate decarboxylase of ...
Archaeoglobus fulgidus
Arch. Microbiol.
182
414-420
2004
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1
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664936
Sender
Characterization of an oxaloac ...
Lactococcus lactis, Lactococcus lactis CRL264
FEBS Lett.
570
217-222
2004
-
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1
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15
1
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3
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5
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1
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2
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1
1
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2
1
1
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1
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15
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1
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3
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1
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2
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1
1
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2
1
1
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650185
Wild
Site-directed sulfhydryl label ...
Klebsiella pneumoniae
Biochemistry
42
11615-11624
2003
-
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1
-
6
-
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1
1
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1
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2
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1
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2
1
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1
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1
1
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6
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1
1
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1
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1
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2
1
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651122
Schmid
Role of conserved residues wit ...
Klebsiella pneumoniae
Eur. J. Biochem.
269
2997-3004
2002
-
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1
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7
-
1
-
1
2
-
1
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2
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1
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2
1
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3
-
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1
2
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1
1
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7
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1
2
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1
2
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1
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1
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2
1
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3
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653877
Trukhina
-
Subcellular localization of ox ...
Zea mays
Russ. J. Plant Physiol.
49
635-640
2002
2
-
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-
1
1
2
2
1
1
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1
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1
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1
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2
1
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1
1
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2
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1
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1
2
2
1
1
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-
-
1
-
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
654328
Labrou
Oxaloacetate decarboxylase fro ...
Pseudomonas stutzeri
Arch. Biochem. Biophys.
365
17-24
1999
-
-
-
-
-
-
13
1
-
2
1
-
-
2
-
-
1
-
-
-
1
1
2
1
-
-
-
-
-
1
-
-
8
-
-
-
-
-
-
-
-
-
-
13
8
1
-
2
1
-
-
-
-
1
-
-
1
1
2
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4276
Jetten
Purification and properties of ...
Azotobacter vinelandii, Corynebacterium glutamicum, Micrococcus luteus
Antonie van Leeuwenhoek
67
221-227
1995
-
1
-
-
-
-
6
1
-
2
2
3
-
4
-
-
1
-
-
-
2
1
6
1
-
-
-
-
1
-
-
2
3
-
-
-
1
-
2
-
-
-
-
6
3
1
-
2
2
3
-
-
-
1
-
-
2
1
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4275
Ivanishchev
-
Investigation of properties of ...
Helianthus annuus
Biokhimiya
58
250-254
1993
-
-
-
-
-
-
2
1
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
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-
2
-
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-
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-
-
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-
2
2
1
1
-
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1
-
-
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-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4283
Guargliardi
Oxalacetate decarboxylase and ...
Saccharolobus solfataricus
Biochim. Biophys. Acta
957
301-311
1988
-
-
-
-
-
-
7
1
-
1
2
1
-
3
-
-
1
1
-
-
1
-
3
1
1
-
1
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
7
-
1
-
1
2
1
-
-
-
1
-
-
1
-
3
1
1
-
1
-
1
1
-
-
-
-
-
-
-
-
4263
Ng
Properties of oxaloacetate dec ...
Veillonella parvula
J. Bacteriol.
150
1252-1258
1982
-
-
-
-
-
1
1
3
-
2
-
1
-
2
-
-
1
1
-
-
1
1
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
2
-
1
-
-
-
1
-
-
1
1
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
4268
Schwitzguebel
-
Oxaloacetate decarboxylase fro ...
Acetobacter aceti
Arch. Microbiol.
124
63-68
1980
-
-
-
-
-
-
-
1
1
-
1
1
-
1
-
-
1
-
-
-
-
2
2
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
1
1
-
-
-
1
-
-
-
2
2
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
4269
Benziman
Purification and regulatory pr ...
Komagataeibacter xylinus
J. Bacteriol.
134
1-9
1978
-
-
-
-
-
-
5
1
1
2
-
1
-
2
-
-
1
-
-
-
2
1
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
5
-
1
1
2
-
1
-
-
-
1
-
-
2
1
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
4270
Wojtczak
Mitochondrial oxaloacetate dec ...
Cavia aperea, Oryctolagus cuniculus, Rattus norvegicus
Biochim. Biophys. Acta
347
168-182
1974
-
-
-
-
-
-
4
1
7
-
-
3
-
4
-
-
1
-
-
6
3
-
6
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
4
1
1
7
-
-
3
-
-
-
1
-
6
3
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
4271
Dean
Oxaloacetate decarboxylases of ...
Rattus norvegicus
Biochem. J.
135
667-672
1973
-
-
-
-
-
-
3
2
3
2
-
1
-
1
-
-
1
-
-
1
-
-
2
-
1
-
-
-
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
2
3
2
-
1
-
-
-
1
-
1
-
-
2
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
4272
Morinaga
-
Studies on oxalacetate carboxy ...
Sus scrofa
Agric. Biol. Chem.
35
1166-1172
1971
-
-
-
-
-
-
8
1
-
4
-
1
-
1
-
-
1
-
-
1
2
-
2
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
1
-
4
-
1
-
-
-
1
-
1
2
-
2
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
4273
Herbert
-
Oxalacetic carboxylase of Micr ...
Micrococcus luteus
Methods Enzymol.
1
753-757
1955
-
-
-
1
-
-
-
-
-
3
-
1
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
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1
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-
-
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1
1
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-
-