Literature summary for 4.1.1.112 extracted from

Studer, R.; Dahinden, P.; Wang, W.W.; Auchli, Y.; Li, X.D.; Dimroth, P.
Crystal structure of the carboxyltransferase domain of the oxaloacetate decarboxylase Na+ pump from Vibrio cholerae (2007), J. Mol. Biol., 367, 547-557.

Search for more literature for 4.1.1.112

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain C43(DE3)	Vibrio cholerae serotype O1

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method using 0.1 M sodium cacodylate (pH 6.5), 0.2 M (NH4)2SO4, 5% (v/v) glycerol and 25% (w/v) PEG 8000	Vibrio cholerae serotype O1

Protein Variants

Protein Variants	Comment	Organism
C148A	74% activity of the wild type enzyme	Vibrio cholerae serotype O1
C148S	54% activity of the wild type enzyme	Vibrio cholerae serotype O1
D17A	inactive	Vibrio cholerae serotype O1
H207I	inactive	Vibrio cholerae serotype O1
H209I	inactive	Vibrio cholerae serotype O1
K178I	inactive	Vibrio cholerae serotype O1
K178R	inactive	Vibrio cholerae serotype O1
M180I	16% activity of the wild type enzyme	Vibrio cholerae serotype O1
Q20L	inactive	Vibrio cholerae serotype O1
R16I	inactive	Vibrio cholerae serotype O1

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Vibrio cholerae serotype O1	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	active site metal ion, 2 mol Zn2+ per mol enzyme	Vibrio cholerae serotype O1

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51000	-	2 * 51000, gel filtration	Vibrio cholerae serotype O1
98300	-	static light scattering	Vibrio cholerae serotype O1
102000	-	gel filtration	Vibrio cholerae serotype O1

Organism

Organism	UniProt	Comment	Textmining
Vibrio cholerae serotype O1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA-agarose column chromatography	Vibrio cholerae serotype O1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Oxaloacetate	-	Vibrio cholerae serotype O1	Pyruvate + CO2	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 51000, gel filtration	Vibrio cholerae serotype O1

Synonyms

Synonyms	Comment	Organism
oxaloacetate carboxylase Na+ pump	membrane-bound multiprotein complex that couples oxaloacetate decarboxylation to sodium ion transport across the membrane	Vibrio cholerae serotype O1

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Release 2023.1 (January 2023)