Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
A383C | beta subunit, very low activity, low activity after modification with 2-aminoethyl methanethiosulfonate | Klebsiella pneumoniae |
G377C | beta subunit, no activity even after modification with 2-aminoethyl methanethiosulfonate | Klebsiella pneumoniae |
G380C | beta subunit, very low activity, low activity after modification with 2-aminoethyl methanethiosulfonate | Klebsiella pneumoniae |
additional information | several other mutant with reduced activity | Klebsiella pneumoniae |
R389C | beta subunit, increased pH-optimum, reduced activity, activity and pH-optimum can be restored to wild type value by modification with 2-aminoethyl methanethiosulfonate | Klebsiella pneumoniae |
S382C | beta subunit, no activity even after modification with 2-aminoethyl methanethiosulfonate | Klebsiella pneumoniae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit | Klebsiella pneumoniae | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | binds to the gamma subunit | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Oxaloacetate | Klebsiella pneumoniae | one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction | Pyruvate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Oxaloacetate | - |
Klebsiella pneumoniae | Pyruvate + CO2 | - |
? | |
Oxaloacetate | one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction | Klebsiella pneumoniae | Pyruvate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | alpha, beta, gamma | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | binds to the C-terminal domain of the alpha-subunit | Klebsiella pneumoniae |