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Literature summary for 4.1.1.111 extracted from
- The structure, function and properties of sirohaem decarboxylase - an enzyme with structural homology to a transcription factor family that is part of the alternative haem biosynthesis pathway (2014), Mol. Microbiol., 93, 247-261 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Methanosarcina barkeri |
| expressed in Escherichia coli BL21(DE3) cells | Desulfovibrio desulfuricans |
| expressed in Escherichia coli BL21(DE3) cells | Desulfovibrio vulgaris |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M sodium acetate pH 4.5, 30% (w/v) PEG 4000, 0.01 M BaCl2 | Desulfovibrio desulfuricans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| siroheme | - |
Desulfovibrio desulfuricans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01174 | - |
siroheme | pH and temperature not specified in the publication | Desulfovibrio desulfuricans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
calculated from amino acid sequence | Desulfovibrio desulfuricans |
| 40000 | - |
gel filtration | Methanosarcina barkeri |
| 40000 | - |
gel filtration | Desulfovibrio desulfuricans |
| 40000 | - |
gel filtration | Desulfovibrio vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| siroheme | Methanosarcina barkeri | - |
12,18-didecarboxysiroheme + CO2 | - |
? | |
| siroheme | Desulfovibrio desulfuricans | - |
12,18-didecarboxysiroheme + CO2 | - |
? | |
| siroheme | Desulfovibrio vulgaris | - |
12,18-didecarboxysiroheme + CO2 | - |
? | |
| siroheme | Desulfovibrio desulfuricans ATCC27774 | - |
12,18-didecarboxysiroheme + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfovibrio desulfuricans | - |
- |
- |
| Desulfovibrio desulfuricans ATCC27774 | - |
- |
- |
| Desulfovibrio vulgaris | - |
- |
- |
| Methanosarcina barkeri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose column chromatography and Superdex 200 gel filtration | Methanosarcina barkeri |
| Ni-Sepharose column chromatography and Superdex 200 gel filtration | Desulfovibrio desulfuricans |
| Ni-Sepharose column chromatography and Superdex 200 gel filtration | Desulfovibrio vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| siroheme | - |
Methanosarcina barkeri | 12,18-didecarboxysiroheme + CO2 | - |
? | |
| siroheme | - |
Desulfovibrio desulfuricans | 12,18-didecarboxysiroheme + CO2 | - |
? | |
| siroheme | - |
Desulfovibrio vulgaris | 12,18-didecarboxysiroheme + CO2 | - |
? | |
| siroheme | - |
Desulfovibrio desulfuricans ATCC27774 | 12,18-didecarboxysiroheme + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | - |
Methanosarcina barkeri |
| heterodimer | - |
Desulfovibrio desulfuricans |
| heterodimer | - |
Desulfovibrio vulgaris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AhbA | subunit | Methanosarcina barkeri |
| AhbA | subunit | Desulfovibrio desulfuricans |
| AhbA | subunit | Desulfovibrio vulgaris |
| AhbB | subunit | Methanosarcina barkeri |
| AhbB | subunit | Desulfovibrio desulfuricans |
| AhbB | subunit | Desulfovibrio vulgaris |
| sirohaem decarboxylase | - |
Methanosarcina barkeri |
| sirohaem decarboxylase | - |
Desulfovibrio desulfuricans |
| sirohaem decarboxylase | - |
Desulfovibrio vulgaris |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00177 | - |
siroheme | pH and temperature not specified in the publication | Desulfovibrio desulfuricans | |
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