BRENDA - Enzyme Database show
show all sequences of 4.1.1.11

Chemoinformatic identification of novel inhibitors against Mycobacterium tuberculosis L-aspartate alpha-decarboxylase

Sharma, R.; Kothapalli, R.; Van Dongen, A.M.; Swaminathan, K.; PLoS ONE 7, e33521 (2012)

Data extracted from this reference:

Application
Application
Commentary
Organism
drug development
the enzyme is a good drug target
Mycobacterium tuberculosis
Inhibitors
Inhibitors
Commentary
Organism
Structure
(2S,3R,4S,5S)-2,3,4,6-tetrahydroxy-5-mercaptohexanal
ZINC03871163
Mycobacterium tuberculosis
(2S,3S,4R,5R)tetrahydro-2H-pyran2,3,4,5-tetraol
ZINC03606295
Mycobacterium tuberculosis
(2S,3S,4R,5S)2,5bis(hydroxymethyl)tetrahydrofuran-2,3,4-triol
ZINC03830875
Mycobacterium tuberculosis
(2S,3S,4S,5R)-2(hydroxymethyl) tetrahydro-2H-pyran-2,3,4,5-tetraol
ZINC03830878
Mycobacterium tuberculosis
(S)-5-acetoxy-4-methylpentanoate
ZINC02036492
Mycobacterium tuberculosis
(S)-thiazolidin-3-ium-4-carboxylate
ZINC00967474
Mycobacterium tuberculosis
1H-pyrazolo[3,4-d]pyrimidin-4(7H)-one
ZINC05177572
Mycobacterium tuberculosis
3-amino-4-(propylamino)cyclobutane-1 2-dione
LIGAND10436
Mycobacterium tuberculosis
additional information
potential inhibitor molecule docking using the enzyme's crystal structure, overview
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartate
Mycobacterium tuberculosis
-
beta-alanine + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
-
gene panD
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate
-
728571
Mycobacterium tuberculosis
beta-alanine + CO2
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
pyruvoyl cofactor
dependent on
Mycobacterium tuberculosis
Application (protein specific)
Application
Commentary
Organism
drug development
the enzyme is a good drug target
Mycobacterium tuberculosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyruvoyl cofactor
dependent on
Mycobacterium tuberculosis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(2S,3R,4S,5S)-2,3,4,6-tetrahydroxy-5-mercaptohexanal
ZINC03871163
Mycobacterium tuberculosis
(2S,3S,4R,5R)tetrahydro-2H-pyran2,3,4,5-tetraol
ZINC03606295
Mycobacterium tuberculosis
(2S,3S,4R,5S)2,5bis(hydroxymethyl)tetrahydrofuran-2,3,4-triol
ZINC03830875
Mycobacterium tuberculosis
(2S,3S,4S,5R)-2(hydroxymethyl) tetrahydro-2H-pyran-2,3,4,5-tetraol
ZINC03830878
Mycobacterium tuberculosis
(S)-5-acetoxy-4-methylpentanoate
ZINC02036492
Mycobacterium tuberculosis
(S)-thiazolidin-3-ium-4-carboxylate
ZINC00967474
Mycobacterium tuberculosis
1H-pyrazolo[3,4-d]pyrimidin-4(7H)-one
ZINC05177572
Mycobacterium tuberculosis
3-amino-4-(propylamino)cyclobutane-1 2-dione
LIGAND10436
Mycobacterium tuberculosis
additional information
potential inhibitor molecule docking using the enzyme's crystal structure, overview
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartate
Mycobacterium tuberculosis
-
beta-alanine + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate
-
728571
Mycobacterium tuberculosis
beta-alanine + CO2
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
L-aspartate alpha-decarboxylase belongs to a class of pyruvoyl dependent enzymes
Mycobacterium tuberculosis
metabolism
L-aspartate alpha-decarboxylase catalyzes the conversion of aspartate to beta-alanine in the pantothenate pathway, which is critical for the growth of Mycobacterium tuberculosis
Mycobacterium tuberculosis
additional information
the enzyme active site is formed by the interface of a dimer, with relatively small volume. This cleft can support only molecules of relatively small size
Mycobacterium tuberculosis
General Information (protein specific)
General Information
Commentary
Organism
evolution
L-aspartate alpha-decarboxylase belongs to a class of pyruvoyl dependent enzymes
Mycobacterium tuberculosis
metabolism
L-aspartate alpha-decarboxylase catalyzes the conversion of aspartate to beta-alanine in the pantothenate pathway, which is critical for the growth of Mycobacterium tuberculosis
Mycobacterium tuberculosis
additional information
the enzyme active site is formed by the interface of a dimer, with relatively small volume. This cleft can support only molecules of relatively small size
Mycobacterium tuberculosis
Other publictions for EC 4.1.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749207
Zhang
-
Glu56Ser mutation improves th ...
Bacillus subtilis, Bacillus subtilis 168, Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025, Escherichia coli, Lactobacillus plantarum, Lactobacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1
Process Biochem.
70
117-123
2018
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3
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3
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7
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11
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4
3
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4
3
3
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3
3
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3
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7
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4
3
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6
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7
4
3
3
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3
3
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4
-
10
10
-
-
-
746598
Gopal
In vivo-selected pyrazinoic a ...
Mycobacterium tuberculosis
ACS Infect. Dis.
3
492-501
2017
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-
18
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5
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1
1
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-
746859
Pei
Molecular engineering of L-as ...
Bacillus subtilis 168, Bacillus subtilis, Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025, Escherichia coli, Escherichia coli K-12 / DH5alpha
Appl. Microbiol. Biotechnol.
101
6015-6021
2017
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3
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2
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2
6
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6
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10
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3
3
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12
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3
2
6
3
3
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1
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1
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6
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6
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3
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12
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3
2
6
3
3
-
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3
3
747128
Arnott
The mechanism of regulation o ...
Escherichia coli
Biochemistry
56
4931-4939
2017
1
1
1
1
4
-
2
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1
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1
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1
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1
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4
4
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747799
Gliessman
Pantothenate auxotrophy in Zy ...
Escherichia coli, no activity in Zymomonas mobilis, no activity in Zymomonas mobilis ZM4 / ATCC 31821 / CP4
FEMS Microbiol. Lett.
364
fnx113
2017
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1
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7
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747435
Stuecker
Phylogenetic and amino acid c ...
Bacillus halodurans, Bacillus halodurans ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125, Bordetella pertussis, Bordetella pertussis Tohama I / ATCC BAA-589 / NCTC 13251, Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025, Helicobacter pylori, Helicobacter pylori 26695, Klebsiella pneumoniae, Klebsiella pneumoniae ATCC 700721 / MGH 78578, Legionella pneumophila, Magnetospirillum magneticum, Magnetospirillum magneticum AMB-1 / ATCC 700264, Moorella thermoacetica, Moorella thermoacetica ATCC 39073 / JCM 9320, Neisseria gonorrhoeae, Neisseria gonorrhoeae ATCC 700825 / FA 1090, Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1, Ralstonia solanacearum, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
BMC Res. Notes
8
354
2015
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12
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1
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22
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24
-
12
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1
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22
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12
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22
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15
15
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747497
Monteiro
The structure of the PanD/Pan ...
Escherichia coli
Chem. Biol.
22
492-503
2015
2
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1
1
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2
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4
4
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747593
Deng
Characterization of L-asparta ...
Bacillus subtilis 168, Bacillus subtilis, Corynebacterium glutamicum, Corynebacterium glutamicum DSM 20300, Escherichia coli
Chin. J. Biotechnol.
31
1184-1193
2015
1
3
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5
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7
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3
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3
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5
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2
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2
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749304
Dai
Aspartate decarboxylase is re ...
Bombyx mori
Sci. Rep.
5
10885
2015
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1
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1
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5
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4
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1
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3
3
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726592
Webb
-
Threonine 57 is required for t ...
Escherichia coli
Acta Crystallogr. Sect. D
70
1166-1172
2014
1
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1
7
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1
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1
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7
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1
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3
3
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727234
Cui
Significance of Arg3, Arg54, a ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Biotechnol. Lett.
36
121-126
2014
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1
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10
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2
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10
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1
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5
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4
1
1
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1
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1
1
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727235
Shen
Synthesis of beta-alanine from ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Biotechnol. Lett.
36
1681-1686
2014
-
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1
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1
1
1
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1
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1
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727784
Wang
beta-Alanine biosynthesis in M ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
J. Bacteriol.
196
2869-2875
2014
-
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1
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747380
Shen
Synthesis of beta-alanine fro ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Biotechnol. Lett.
36
1681-1686
2014
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1
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748086
Tomita
An archaeal glutamate decarbo ...
Thermococcus kodakarensis, Thermococcus kodakarensis ATCC BAA-918 / JCM 12380 / KOD1
J. Bacteriol.
196
1222-1230
2014
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1
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2
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3
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744560
Lee
-
Gene expression and character ...
Pyrococcus furiosus
Biotechnol. Bioprocess Eng.
18
375-381
2013
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726617
Webb
Structure of Escherichia coli ...
Escherichia coli
Acta Crystallogr. Sect. F
68
414-417
2012
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1
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726873
Monteiro
Formation of a heterooctameric ...
Escherichia coli
Biochem. Biophys. Res. Commun.
426
350-355
2012
2
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1
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727622
Liu
Cysteine sulfinic acid decarbo ...
Aedes aegypti, Drosophila melanogaster
Insect Biochem. Mol. Biol.
42
396-403
2012
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1
1
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2
728234
Stuecker
-
PanM, an acetyl-coenzyme a sen ...
Salmonella enterica, Salmonella enterica DM10310
mBio
3
e00158-12
2012
2
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2
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728266
Nozaki
An activator for pyruvoyl-depe ...
Escherichia coli, Escherichia coli MG1655
MicrobiologyOpen
1
298-310
2012
1
-
1
-
1
-
-
-
-
-
-
2
-
26
-
1
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
1
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
3
3
-
-
-
728327
Stuecker
The missing link in coenzyme A ...
Corynebacterium glutamicum, Salmonella enterica
Mol. Microbiol.
84
608-619
2012
2
-
2
-
1
-
-
-
-
-
1
2
-
7
-
1
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
2
-
-
-
2
-
2
2
-
1
-
-
-
-
-
-
-
1
2
-
-
1
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
728571
Sharma
Chemoinformatic identification ...
Mycobacterium tuberculosis
PLoS ONE
7
e33521
2012
-
1
-
-
-
-
9
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
728583
Sharma
Validation of drug-like inhibi ...
Mycobacterium tuberculosis
PLoS ONE
7
e45947
2012
-
-
1
-
-
-
12
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
12
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714398
Lomakin
Mechanical properties of the b ...
Tribolium castaneum
Biomacromolecules
12
321-335
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
714741
de Villiers
3-Fluoroaspartate and pyruvoyl ...
Escherichia coli, Mycobacterium tuberculosis
Chemistry
16
10030-10041
2010
2
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704491
Arakane
Molecular and functional analy ...
Tribolium castaneum, Tribolium castaneum GA-1
J. Biol. Chem.
284
16584-16594
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
706856
Fouad
Transplastomic expression of b ...
Escherichia coli
Transgenic Res.
18
707-718
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682386
Fouad
Expression of bacterial L-aspa ...
Escherichia coli
Plant Mol. Biol.
60
495-505
2006
-
-
1
-
1
-
-
-
-
-
1
1
-
4
-
-
1
-
-
-
-
-
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
682753
Gopalan
Crystal structure of uncleaved ...
Mycobacterium tuberculosis
Proteins
65
796-802
2006
-
1
1
1
-
-
-
-
-
-
-
1
-
5
-
1
1
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665041
Phillips
The Drosophila black enigma: T ...
Drosophila melanogaster
Gene
351
131-142
2005
-
-
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666021
Lee
Crystal structure of the Schif ...
Helicobacter pylori
J. Mol. Biol.
340
1-7
2004
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650998
Schmitzberger
Structural constraints on prot ...
Escherichia coli
EMBO J.
22
6193-6204
2003
-
-
1
1
6
-
-
-
-
-
1
1
-
2
-
1
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
6
-
-
-
-
-
-
-
1
1
-
-
1
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649196
Kwon
Crystallization and preliminar ...
Helicobacter pylori
Acta Crystallogr. Sect. D
58
861-863
2002
-
-
1
1
-
-
-
-
1
-
-
1
-
7
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653749
Chopra
Expression, purification, and ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Protein Expr. Purif.
25
533-540
2002
-
1
1
-
-
-
-
1
1
-
2
2
-
162
-
1
1
-
-
-
1
-
4
1
1
-
-
1
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
1
-
2
2
-
-
1
1
-
-
1
-
4
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
650836
Saldanha
-
Identification of Tyr58 as the ...
Escherichia coli
Chem. Commun. (Camb.)
2001
1760-1761
2001
-
-
1
1
-
-
-
-
-
-
1
1
-
1
-
1
1
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
1
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
649350
Dusch
Expression of the Corynebacter ...
Corynebacterium glutamicum, Escherichia coli, Escherichia coli MG1655
Appl. Environ. Microbiol.
65
1530-1539
1999
-
-
2
1
2
-
-
-
-
-
1
3
-
5
-
2
-
-
-
-
1
-
6
2
1
-
-
-
-
-
-
1
-
-
-
-
-
2
1
1
2
-
-
-
-
-
-
-
1
3
-
-
2
-
-
-
1
-
6
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3949
Albert
Crystal structure of aspartate ...
Escherichia coli
Nat. Struct. Biol.
5
289-293
1998
1
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3950
Ramjee
Escherichia coli L-aspartate a ...
Escherichia coli
Biochem. J.
323
661-669
1997
1
-
1
-
-
-
-
1
-
-
3
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3947
Williamson
L-Aspartate alpha-decarboxylas ...
Escherichia coli, Escherichia coli B / ATCC 11303
Methods Enzymol.
113
589-595
1985
1
-
-
-
-
-
12
1
-
-
1
-
-
177
-
-
1
-
-
-
1
-
2
-
1
1
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
12
-
1
-
-
1
-
-
-
-
1
-
-
1
-
2
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
3948
Williamson
Purification and properties of ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
254
8074-8082
1979
1
-
-
-
-
-
12
1
-
-
1
-
-
178
-
-
1
-
-
-
1
-
2
-
1
1
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
12
-
1
-
-
1
-
-
-
-
1
-
-
1
-
2
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-