BRENDA - Enzyme Database show
show all sequences of 4.1.1.11

Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli

Dusch, N.; Pühler, A.; Kalinowski, J.; Appl. Environ. Microbiol. 65, 1530-1539 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
panD gene, overexpression in Corynebacterium glutamicum with 4fold increased enzyme activity and in Escherichia coli with 3fold increased enzyme activity
Escherichia coli
panD gene, sequencing, overexpression in Corynebacterium glutamicum with 288fold increased enzyme activity and in Escherichia coli with 41fold increased enzyme activity
Corynebacterium glutamicum
Crystallization (Commentary)
Crystallization
Organism
-
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
additional information
panD insertion mutant ND2 completely lacks enzyme activity and exhibits beta-alanine auxotrophy
Corynebacterium glutamicum
additional information
panD mutants
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14100
-
x * 14100, pi-protein, calculated from the amino acid sequence
Corynebacterium glutamicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartate
Corynebacterium glutamicum
essential for beta-alanine synthesis, involved in pantothenate biosynthesis
beta-alanine + CO2
-
Corynebacterium glutamicum
?
L-aspartate
Escherichia coli
involved in pantothenate biosynthesis
beta-alanine + CO2
-
Escherichia coli
?
L-aspartate
Escherichia coli MG1655
involved in pantothenate biosynthesis
beta-alanine + CO2
-
Escherichia coli MG1655
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corynebacterium glutamicum
Q9X4N0
strain ATCC 13032
-
Escherichia coli
P0A790
-
-
Escherichia coli MG1655
P0A790
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
the panD gene product is efficiently proteolytically processed into two subunits, an 11 kDa alpha-subunit and a 2.7 kDa beta-subunit, Gly-24/Ser-25 may be the processing site of the initially translated pi-protein
Corynebacterium glutamicum
proteolytic modification
panD is initially translated as inactive precursor pi-protein which is slowly proteolytically cleaved at a specific Gly-Ser bond producing two dissimilar subunits, autocatalytic mechanism
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Corynebacterium glutamicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate
-
649350
Corynebacterium glutamicum
beta-alanine + CO2
-
649350
Corynebacterium glutamicum
?
L-aspartate
alpha-decarboxylation
649350
Escherichia coli
beta-alanine + CO2
-
649350
Escherichia coli
?
L-aspartate
essential for beta-alanine synthesis, involved in pantothenate biosynthesis
649350
Corynebacterium glutamicum
beta-alanine + CO2
-
649350
Corynebacterium glutamicum
?
L-aspartate
involved in pantothenate biosynthesis
649350
Escherichia coli
beta-alanine + CO2
-
649350
Escherichia coli
?
L-aspartate
alpha-decarboxylation
649350
Escherichia coli MG1655
beta-alanine + CO2
-
649350
Escherichia coli MG1655
?
L-aspartate
involved in pantothenate biosynthesis
649350
Escherichia coli MG1655
beta-alanine + CO2
-
649350
Escherichia coli MG1655
?
Subunits
Subunits
Commentary
Organism
?
x * 14100, pi-protein, calculated from the amino acid sequence
Corynebacterium glutamicum
tetramer
active enzyme is a tetramer composed of three alpha- and beta-subunits and an incompletely processed pi-protein
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Corynebacterium glutamicum
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
requires pyruvate as a covalently bound, catalytically active prosthetic group, pyruvoyl-dependent enzyme, the pyruvoyl group is generated at a specific internal serine residue of the inactive pi-protein with the coincident cleavage of the Gly-Ser bond
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
panD gene, overexpression in Corynebacterium glutamicum with 4fold increased enzyme activity and in Escherichia coli with 3fold increased enzyme activity
Escherichia coli
panD gene, sequencing, overexpression in Corynebacterium glutamicum with 288fold increased enzyme activity and in Escherichia coli with 41fold increased enzyme activity
Corynebacterium glutamicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
requires pyruvate as a covalently bound, catalytically active prosthetic group, pyruvoyl-dependent enzyme, the pyruvoyl group is generated at a specific internal serine residue of the inactive pi-protein with the coincident cleavage of the Gly-Ser bond
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
panD insertion mutant ND2 completely lacks enzyme activity and exhibits beta-alanine auxotrophy
Corynebacterium glutamicum
additional information
panD mutants
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14100
-
x * 14100, pi-protein, calculated from the amino acid sequence
Corynebacterium glutamicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartate
Corynebacterium glutamicum
essential for beta-alanine synthesis, involved in pantothenate biosynthesis
beta-alanine + CO2
-
Corynebacterium glutamicum
?
L-aspartate
Escherichia coli
involved in pantothenate biosynthesis
beta-alanine + CO2
-
Escherichia coli
?
L-aspartate
Escherichia coli MG1655
involved in pantothenate biosynthesis
beta-alanine + CO2
-
Escherichia coli MG1655
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
the panD gene product is efficiently proteolytically processed into two subunits, an 11 kDa alpha-subunit and a 2.7 kDa beta-subunit, Gly-24/Ser-25 may be the processing site of the initially translated pi-protein
Corynebacterium glutamicum
proteolytic modification
panD is initially translated as inactive precursor pi-protein which is slowly proteolytically cleaved at a specific Gly-Ser bond producing two dissimilar subunits, autocatalytic mechanism
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate
-
649350
Corynebacterium glutamicum
beta-alanine + CO2
-
649350
Corynebacterium glutamicum
?
L-aspartate
alpha-decarboxylation
649350
Escherichia coli
beta-alanine + CO2
-
649350
Escherichia coli
?
L-aspartate
essential for beta-alanine synthesis, involved in pantothenate biosynthesis
649350
Corynebacterium glutamicum
beta-alanine + CO2
-
649350
Corynebacterium glutamicum
?
L-aspartate
involved in pantothenate biosynthesis
649350
Escherichia coli
beta-alanine + CO2
-
649350
Escherichia coli
?
L-aspartate
alpha-decarboxylation
649350
Escherichia coli MG1655
beta-alanine + CO2
-
649350
Escherichia coli MG1655
?
L-aspartate
involved in pantothenate biosynthesis
649350
Escherichia coli MG1655
beta-alanine + CO2
-
649350
Escherichia coli MG1655
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 14100, pi-protein, calculated from the amino acid sequence
Corynebacterium glutamicum
tetramer
active enzyme is a tetramer composed of three alpha- and beta-subunits and an incompletely processed pi-protein
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Corynebacterium glutamicum
Other publictions for EC 4.1.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749207
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Glu56Ser mutation improves th ...
Bacillus subtilis, Bacillus subtilis 168, Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025, Escherichia coli, Lactobacillus plantarum, Lactobacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1
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3
-
3
-
-
-
-
-
-
7
-
11
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4
3
-
-
-
6
-
7
4
3
3
-
-
3
3
-
-
-
4
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
7
-
-
4
3
-
-
6
-
7
4
3
3
-
-
3
3
-
4
-
10
10
-
-
-
746598
Gopal
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3
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-
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-
-
18
-
-
-
-
-
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-
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5
-
-
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-
-
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18
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-
-
-
-
-
-
-
-
-
1
1
-
-
-
746859
Pei
Molecular engineering of L-as ...
Bacillus subtilis 168, Bacillus subtilis, Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025, Escherichia coli, Escherichia coli K-12 / DH5alpha
Appl. Microbiol. Biotechnol.
101
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2017
-
-
3
-
2
-
2
6
-
-
-
6
-
10
-
3
3
-
-
-
-
-
12
-
3
2
6
3
3
-
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1
-
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3
1
-
2
-
-
2
-
6
-
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6
-
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3
3
-
-
-
-
12
-
3
2
6
3
3
-
-
-
-
-
-
-
3
3
747128
Arnott
The mechanism of regulation o ...
Escherichia coli
Biochemistry
56
4931-4939
2017
1
1
1
1
4
-
2
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-
-
-
1
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2
-
1
1
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1
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1
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1
1
1
1
1
4
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2
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1
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1
1
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-
1
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-
-
-
-
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-
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-
4
4
-
-
-
747799
Gliessman
Pantothenate auxotrophy in Zy ...
Escherichia coli, no activity in Zymomonas mobilis, no activity in Zymomonas mobilis ZM4 / ATCC 31821 / CP4
FEMS Microbiol. Lett.
364
fnx113
2017
-
-
1
-
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1
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7
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1
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1
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747435
Stuecker
Phylogenetic and amino acid c ...
Bacillus halodurans, Bacillus halodurans ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125, Bordetella pertussis, Bordetella pertussis Tohama I / ATCC BAA-589 / NCTC 13251, Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025, Helicobacter pylori, Helicobacter pylori 26695, Klebsiella pneumoniae, Klebsiella pneumoniae ATCC 700721 / MGH 78578, Legionella pneumophila, Magnetospirillum magneticum, Magnetospirillum magneticum AMB-1 / ATCC 700264, Moorella thermoacetica, Moorella thermoacetica ATCC 39073 / JCM 9320, Neisseria gonorrhoeae, Neisseria gonorrhoeae ATCC 700825 / FA 1090, Pseudomonas aeruginosa, Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1, Ralstonia solanacearum, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
BMC Res. Notes
8
354
2015
-
-
12
-
1
-
-
-
-
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22
-
24
-
12
-
-
-
1
-
-
22
-
-
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-
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3
-
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12
3
-
1
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22
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12
-
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1
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22
-
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-
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15
15
-
-
-
747497
Monteiro
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Escherichia coli
Chem. Biol.
22
492-503
2015
2
-
-
1
1
-
2
-
-
-
-
-
-
1
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1
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1
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2
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1
1
1
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2
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1
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4
4
-
-
-
747593
Deng
Characterization of L-asparta ...
Bacillus subtilis 168, Bacillus subtilis, Corynebacterium glutamicum, Corynebacterium glutamicum DSM 20300, Escherichia coli
Chin. J. Biotechnol.
31
1184-1193
2015
1
3
-
-
-
-
-
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5
-
7
-
3
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3
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5
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2
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2
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1
3
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5
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3
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3
-
5
-
2
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-
2
-
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-
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749304
Dai
Aspartate decarboxylase is re ...
Bombyx mori
Sci. Rep.
5
10885
2015
-
-
1
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1
-
-
-
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1
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5
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4
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1
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1
1
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1
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1
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4
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1
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3
3
-
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726592
Webb
-
Threonine 57 is required for t ...
Escherichia coli
Acta Crystallogr. Sect. D
70
1166-1172
2014
1
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1
7
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1
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1
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1
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1
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7
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1
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1
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1
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-
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3
3
-
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727234
Cui
Significance of Arg3, Arg54, a ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Biotechnol. Lett.
36
121-126
2014
-
-
1
-
10
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2
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4
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1
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5
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1
1
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1
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1
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10
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2
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1
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5
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4
1
1
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1
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1
1
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-
727235
Shen
Synthesis of beta-alanine from ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Biotechnol. Lett.
36
1681-1686
2014
-
-
1
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1
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1
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3
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1
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1
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1
1
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1
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1
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1
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3
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4
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1
1
1
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1
1
1
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727784
Wang
beta-Alanine biosynthesis in M ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
J. Bacteriol.
196
2869-2875
2014
-
-
1
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1
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2
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6
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1
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1
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1
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4
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1
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1
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1
1
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747380
Shen
Synthesis of beta-alanine fro ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Biotechnol. Lett.
36
1681-1686
2014
-
-
1
-
1
-
-
-
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2
-
4
-
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1
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-
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1
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2
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1
1
3
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1
1
2
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1
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1
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2
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1
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1
-
2
-
1
1
3
-
1
1
2
-
-
-
-
-
-
-
748086
Tomita
An archaeal glutamate decarbo ...
Thermococcus kodakarensis, Thermococcus kodakarensis ATCC BAA-918 / JCM 12380 / KOD1
J. Bacteriol.
196
1222-1230
2014
-
-
1
-
1
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1
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4
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7
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1
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6
2
1
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1
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1
1
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1
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4
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1
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6
2
1
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1
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-
3
3
-
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744560
Lee
-
Gene expression and character ...
Pyrococcus furiosus
Biotechnol. Bioprocess Eng.
18
375-381
2013
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