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Literature summary for 4.1.1.107 extracted from
- Biochemical identification of residues that discriminate between 3,4-dihydroxyphenylalanine decarboxylase and 3,4-dihydroxyphenylacetaldehyde synthase-mediated reactions (2017), Insect Biochem. Mol. Biol., 91, 34-43 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2566 cells | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C194S | the mutant shows about wild type activity | Drosophila melanogaster |
| N192H | the mutant shows increased L-dopa decarboxylase activity compared to the wild type enzyme | Drosophila melanogaster |
| Y80F | the mutant shows about wild type activity | Drosophila melanogaster |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.88 | - |
L-Dopa | wild type enzyme, pH and temperature not specified in the publication | Drosophila melanogaster |  |
| 1.23 | - |
L-Dopa | mutant enzyme N192H, pH and temperature not specified in the publication | Drosophila melanogaster | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Dopa + O2 + H2O | Drosophila melanogaster | - |
3,4-dihydroxyphenylacetaldehyde + CO2 + NH3 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | Q9VIZ9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anion exchange column chromatography and gel filtration | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Dopa + O2 + H2O | - |
Drosophila melanogaster | 3,4-dihydroxyphenylacetaldehyde + CO2 + NH3 + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHPA synthase | - |
Drosophila melanogaster |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Drosophila melanogaster | |
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