Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-tryptophan | non-competitive inhibitor | Catharanthus roseus | |
additional information | not inhibited by CO2 | Catharanthus roseus | |
tryptamine | competitive inhibitor | Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
L-tryptophan | at pH 7.5, temperature not specified in the publication | Catharanthus roseus | |
1.3 | - |
5-hydroxy-L-tryptophan | at pH 7.5, temperature not specified in the publication | Catharanthus roseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
gel filtration | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan | Catharanthus roseus | - |
tryptamine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephacel column chromatography, hydroxylapatite column chromatography, AcA-34 column chromatography, TSK-4000 gel filtration, Mono Q column chromatography, and TSK-3000 gel filtration | Catharanthus roseus |
Storage Stability | Organism |
---|---|
0°C, purified enzyme at pH 7.5, 3 weeks, 90% loss of activity | Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxy-L-tryptophan | - |
Catharanthus roseus | 5-hydroxytryptamine + CO2 | - |
? | |
L-tryptophan | - |
Catharanthus roseus | tryptamine + CO2 | - |
? | |
additional information | L-phenylalanine, L-tyrosine and DOPA are not decarboxylated by the enzyme | Catharanthus roseus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 54000, SDS-PAGE | Catharanthus roseus |
Synonyms | Comment | Organism |
---|---|---|
TDC | - |
Catharanthus roseus |
Tryptophan decarboxylase | - |
Catharanthus roseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.33 | - |
L-tryptophan | at pH 7.5, temperature not specified in the publication | Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Catharanthus roseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Catharanthus roseus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
tryptamine | at pH 7.5, temperature not specified in the publication | Catharanthus roseus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Catharanthus roseus | isoelectric focusing | - |
5.9 |