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Literature summary for 4.1.1.105 extracted from

  • Noe, W.; Mollenschott, C.; Berlin, J.
    Tryptophan decarboxylase from Catharanthus roseus cell suspension cultures purification, molecular and kinetic data of the homogenous protein (1984), Plant Mol. Biol., 3, 281-288 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-tryptophan non-competitive inhibitor Catharanthus roseus
additional information not inhibited by CO2 Catharanthus roseus
tryptamine competitive inhibitor Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
L-tryptophan at pH 7.5, temperature not specified in the publication Catharanthus roseus
1.3
-
5-hydroxy-L-tryptophan at pH 7.5, temperature not specified in the publication Catharanthus roseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
gel filtration Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan Catharanthus roseus
-
tryptamine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sephacel column chromatography, hydroxylapatite column chromatography, AcA-34 column chromatography, TSK-4000 gel filtration, Mono Q column chromatography, and TSK-3000 gel filtration Catharanthus roseus

Storage Stability

Storage Stability Organism
0Ā°C, purified enzyme at pH 7.5, 3 weeks, 90% loss of activity Catharanthus roseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxy-L-tryptophan
-
Catharanthus roseus 5-hydroxytryptamine + CO2
-
?
L-tryptophan
-
Catharanthus roseus tryptamine + CO2
-
?
additional information L-phenylalanine, L-tyrosine and DOPA are not decarboxylated by the enzyme Catharanthus roseus ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 54000, SDS-PAGE Catharanthus roseus

Synonyms

Synonyms Comment Organism
TDC
-
Catharanthus roseus
Tryptophan decarboxylase
-
Catharanthus roseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.33
-
L-tryptophan at pH 7.5, temperature not specified in the publication Catharanthus roseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Catharanthus roseus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Catharanthus roseus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.31
-
tryptamine at pH 7.5, temperature not specified in the publication Catharanthus roseus

pI Value

Organism Comment pI Value Maximum pI Value
Catharanthus roseus isoelectric focusing
-
5.9