Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
to 2.45 A resolution. The conformational flexibility of the beta2e-alpha5 loop allows access to the active site. The structure implicates Glu285 as the general base. An about 30-A-long pocket adjacent to the catalytic site may accommodate the isoprenoid tail of the substrate needed for ubiquinone biosynthesis in yeast | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E285A | loss of catalytic activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
4-coumarate | pH 6.5, 30°C | Saccharomyces cerevisiae | |
0.92 | - |
ferulate | pH 6.5, 30°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q03034 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumarate | - |
Saccharomyces cerevisiae | 4-vinylphenol + CO2 | - |
? | |
ferulate | - |
Saccharomyces cerevisiae | 4-vinylguaiacol + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDC1 | - |
Saccharomyces cerevisiae |
ubiD | - |
Saccharomyces cerevisiae |