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Literature summary for 4.1.1.101 extracted from

  • Naouri, P.; Chagnaud, P.; Arnaud, A.; Galzy, P.
    Purification and properties of a malolactic enzyme from Leuconostoc oenos ATCC 23278 (1990), J. Basic Microbiol., 30, 577-585.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
citrate competitive Oenococcus oeni
D-Tartrate competitive Oenococcus oeni
dithiothreitol 0.1 mM, 44% residual activity Oenococcus oeni
EDTA 0.1 mM, no residual activity Oenococcus oeni
ethanol competitive Oenococcus oeni
L-lactate noncompetitive Oenococcus oeni
N-bromosuccinimide 0.1 mM, 31% residual activity Oenococcus oeni
succinate competitive Oenococcus oeni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17
-
L-malate pH 6.0, 37°C Oenococcus oeni

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 90% of the activity with Mn2+ Oenococcus oeni
Cu2+ 31% of the activity with Mn2+ Oenococcus oeni
Fe2+ 98% of the activity with Mn2+ Oenococcus oeni
Mg2+ 16% of the activity with Mn2+ Oenococcus oeni
Mn2+ required, Km value 0.017 mM Oenococcus oeni
Zn2+ 29% of the activity with Mn2+ Oenococcus oeni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
2 * 66000, SDS-PAGE Oenococcus oeni
132000
-
gel filtration Oenococcus oeni

Organism

Organism UniProt Comment Textmining
Oenococcus oeni
-
-
-
Oenococcus oeni ATCC 23278
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oenococcus oeni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-malate
-
Oenococcus oeni L-lactate + CO2
-
?
L-malate
-
Oenococcus oeni ATCC 23278 L-lactate + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 66000, SDS-PAGE Oenococcus oeni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Oenococcus oeni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Oenococcus oeni

Cofactor

Cofactor Comment Organism Structure
NAD+ required, Km value 0.044 mM Oenococcus oeni

pI Value

Organism Comment pI Value Maximum pI Value
Oenococcus oeni isoelectric focusing
-
4.3