BRENDA - Enzyme Database show
show all sequences of 4.1.1.101

Purification and properties of a malolactic enzyme from Leuconostoc oenos ATCC 23278

Naouri, P.; Chagnaud, P.; Arnaud, A.; Galzy, P.; J. Basic Microbiol. 30, 577-585 (1990)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
citrate
competitive
Oenococcus oeni
D-Tartrate
competitive
Oenococcus oeni
dithiothreitol
0.1 mM, 44% residual activity
Oenococcus oeni
EDTA
0.1 mM, no residual activity
Oenococcus oeni
ethanol
competitive
Oenococcus oeni
L-lactate
noncompetitive
Oenococcus oeni
N-bromosuccinimide
0.1 mM, 31% residual activity
Oenococcus oeni
succinate
competitive
Oenococcus oeni
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
17
-
L-malate
pH 6.0, 37°C
Oenococcus oeni
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
90% of the activity with Mn2+
Oenococcus oeni
Cu2+
31% of the activity with Mn2+
Oenococcus oeni
Fe2+
98% of the activity with Mn2+
Oenococcus oeni
Mg2+
16% of the activity with Mn2+
Oenococcus oeni
Mn2+
required, Km value 0.017 mM
Oenococcus oeni
Zn2+
29% of the activity with Mn2+
Oenococcus oeni
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66000
-
2 * 66000, SDS-PAGE
Oenococcus oeni
132000
-
gel filtration
Oenococcus oeni
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oenococcus oeni
-
-
-
Oenococcus oeni ATCC 23278
-
-
-
Purification (Commentary)
Commentary
Organism
-
Oenococcus oeni
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-malate
-
734101
Oenococcus oeni
L-lactate + CO2
-
-
-
-
L-malate
-
734101
Oenococcus oeni ATCC 23278
L-lactate + CO2
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 66000, SDS-PAGE
Oenococcus oeni
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Oenococcus oeni
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Oenococcus oeni
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
required, Km value 0.044 mM
Oenococcus oeni
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Oenococcus oeni
isoelectric focusing
-
4.3
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
required, Km value 0.044 mM
Oenococcus oeni
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
citrate
competitive
Oenococcus oeni
D-Tartrate
competitive
Oenococcus oeni
dithiothreitol
0.1 mM, 44% residual activity
Oenococcus oeni
EDTA
0.1 mM, no residual activity
Oenococcus oeni
ethanol
competitive
Oenococcus oeni
L-lactate
noncompetitive
Oenococcus oeni
N-bromosuccinimide
0.1 mM, 31% residual activity
Oenococcus oeni
succinate
competitive
Oenococcus oeni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
17
-
L-malate
pH 6.0, 37°C
Oenococcus oeni
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
90% of the activity with Mn2+
Oenococcus oeni
Cu2+
31% of the activity with Mn2+
Oenococcus oeni
Fe2+
98% of the activity with Mn2+
Oenococcus oeni
Mg2+
16% of the activity with Mn2+
Oenococcus oeni
Mn2+
required, Km value 0.017 mM
Oenococcus oeni
Zn2+
29% of the activity with Mn2+
Oenococcus oeni
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66000
-
2 * 66000, SDS-PAGE
Oenococcus oeni
132000
-
gel filtration
Oenococcus oeni
Purification (Commentary) (protein specific)
Commentary
Organism
-
Oenococcus oeni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-malate
-
734101
Oenococcus oeni
L-lactate + CO2
-
-
-
-
L-malate
-
734101
Oenococcus oeni ATCC 23278
L-lactate + CO2
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 66000, SDS-PAGE
Oenococcus oeni
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Oenococcus oeni
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Oenococcus oeni
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Oenococcus oeni
isoelectric focusing
-
4.3
Other publictions for EC 4.1.1.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748018
Kim
Identification and characteri ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides ATCC 8293
J. Agric. Food Chem.
66
8086-8093
2018
-
-
1
-
-
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4
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1
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1
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1
-
-
747968
Rizk
Impact of inhibitory peptides ...
Oenococcus oeni, Oenococcus oeni Vitilactic F
Int. J. Food Microbiol.
233
90-96
2016
-
1
-
-
-
-
1
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5
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748514
Heo
-
Isolation of indigenous Lacto ...
Lactobacillus plantarum, Lactobacillus plantarum JBE60
Korean J. Microbiol.
51
169-176
2015
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1
-
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2
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733759
Wang
-
Induction, purification and ch ...
Oenococcus oeni, Oenococcus oeni SD-2a
Eur. Food Res. Technol.
239
827-835
2014
-
-
-
-
-
-
-
1
-
5
1
-
1
2
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1
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-
1
1
2
1
1
1
-
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1
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1
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-
-
-
-
-
-
-
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1
-
5
1
-
1
-
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1
-
-
1
1
2
1
1
1
-
-
1
-
-
1
1
-
-
1
-
-
747817
Cappello
Bio-molecular characterisatio ...
Oenococcus oeni
Food Microbiol.
42
142-148
2014
-
1
-
-
-
-
-
-
-
-
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3
-
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1
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-
-
-
-
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-
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748284
Liu
-
Screening of lactic acid bact ...
Oenococcus oeni
J. Chem. Pharm. Res.
6
136-144
2014
-
1
-
-
-
-
-
-
-
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1
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1
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-
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-
-
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-
-
-
-
-
733129
Zhang
Surface display of malolactic ...
Oenococcus oeni
Appl. Biochem. Biotechnol.
169
2350-2361
2013
-
1
-
-
-
-
-
-
2
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6
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2
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1
1
-
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733168
Landete
Malic enzyme and malolactic en ...
Lactobacillus casei
Appl. Environ. Microbiol.
79
5509-5518
2013
-
-
-
-
-
-
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-
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4
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1
1
1
1
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733070
Schuemann
Heterologous expression of Oen ...
Oenococcus oeni
AMB Express
2
19
2012
-
1
-
-
-
-
-
-
-
-
2
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4
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1
1
1
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1
1
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1
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2
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1
1
1
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-
1
1
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1
1
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733686
Miller
Expression of the malolactic e ...
Lactobacillus plantarum
Curr. Microbiol.
62
1682-1688
2011
-
-
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1
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3
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1
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1
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1
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1
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1
2
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2
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733149
Arthurs
Kinetics, stereospecificity, a ...
Lactobacillus collinoides
Appl. Environ. Microbiol.
65
3360-3363
1999
-
-
-
-
-
-
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3
-
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3
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3
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1
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3
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3
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1
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1
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1
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733147
Labarre
Cloning and characterization o ...
Oenococcus oeni
Appl. Environ. Microbiol.
62
1274-1282
1996
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1
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1
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5
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1
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1
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733881
Denayrolles
Functional expression in Sacch ...
Lactococcus lactis
FEMS Microbiol. Lett.
125
37-43
1995
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1
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5
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1
1
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733845
Ansanay
Cloning, sequence and expressi ...
Lactococcus lactis, Lactococcus lactis IL1403
FEBS Lett.
332
74-80
1993
-
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2
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5
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1
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733600
Battermann
-
A comparative study of malolac ...
Lactobacillus casei, Leuconostoc mesenteroides, Leuconostoc mesenteroides B116, Oenococcus oeni
Can. J. Microbiol.
37
211-217
1991
-
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2
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9
6
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4
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3
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3
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6
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4
3
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3
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734101
Naouri
Purification and properties of ...
Oenococcus oeni, Oenococcus oeni ATCC 23278
J. Basic Microbiol.
30
577-585
1990
-
-
-
-
-
-
8
1
-
6
2
-
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3
-
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1
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2
1
1
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1
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1
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1
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1
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8
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1
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6
2
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1
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2
1
1
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1
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1
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734043
Strasser de Saad
Purification and properties of ...
Lactobacillus murinus, Lactobacillus murinus CNRZ 313
J. Appl. Biochem.
6
374-383
1985
-
-
-
-
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2
-
1
2
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6
-
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1
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1
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2
1
1
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1
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1
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1
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2
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1
2
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1
1
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2
1
1
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1
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733143
Spettoli
Properties of malolactic activ ...
Oenococcus oeni, Oenococcus oeni ML34
Appl. Environ. Microbiol.
48
900-901
1984
-
-
-
-
-
-
-
1
-
6
1
-
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3
-
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1
-
-
-
-
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4
-
1
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1
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-
1
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1
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-
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1
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6
1
-
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1
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-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
734116
Caspritz
Malolactic enzyme of Lactobaci ...
Lactobacillus plantarum
J. Biol. Chem.
258
4907-4910
1983
-
-
-
-
-
-
-
1
-
1
2
-
-
3
-
-
1
-
-
-
1
-
1
1
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1
-
1
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1
-
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1
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1
2
-
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1
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1
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1
1
-
-
-
-
-
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1
-
-
-
-
-
-
733142
Lonvaud-Funel
Purification and properties of ...
Leuconostoc mesenteroides
Appl. Environ. Microbiol.
43
357-361
1982
-
-
-
-
-
-
8
1
-
1
1
-
-
2
-
-
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-
1
-
1
-
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-
1
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1
8
1
-
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1
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8
8
1
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1
1
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1
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1
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1
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1
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