Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | competitive | Oenococcus oeni | |
D-Tartrate | competitive | Oenococcus oeni | |
dithiothreitol | 0.1 mM, 44% residual activity | Oenococcus oeni | |
EDTA | 0.1 mM, no residual activity | Oenococcus oeni | |
ethanol | competitive | Oenococcus oeni | |
L-lactate | noncompetitive | Oenococcus oeni | |
N-bromosuccinimide | 0.1 mM, 31% residual activity | Oenococcus oeni | |
succinate | competitive | Oenococcus oeni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
L-malate | pH 6.0, 37°C | Oenococcus oeni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 90% of the activity with Mn2+ | Oenococcus oeni | |
Cu2+ | 31% of the activity with Mn2+ | Oenococcus oeni | |
Fe2+ | 98% of the activity with Mn2+ | Oenococcus oeni | |
Mg2+ | 16% of the activity with Mn2+ | Oenococcus oeni | |
Mn2+ | required, Km value 0.017 mM | Oenococcus oeni | |
Zn2+ | 29% of the activity with Mn2+ | Oenococcus oeni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
2 * 66000, SDS-PAGE | Oenococcus oeni |
132000 | - |
gel filtration | Oenococcus oeni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oenococcus oeni | - |
- |
- |
Oenococcus oeni ATCC 23278 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oenococcus oeni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-malate | - |
Oenococcus oeni | L-lactate + CO2 | - |
? | |
L-malate | - |
Oenococcus oeni ATCC 23278 | L-lactate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 66000, SDS-PAGE | Oenococcus oeni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Oenococcus oeni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Oenococcus oeni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required, Km value 0.044 mM | Oenococcus oeni |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oenococcus oeni | isoelectric focusing | - |
4.3 |